| |
| |
| Preface | |
| |
| |
| |
| Overview of bioseparations engineering | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| What is separated in bioseparation? | |
| |
| |
| |
| Economic importance of bioseparation | |
| |
| |
| |
| Nature of bioseparation | |
| |
| |
| |
| Basis of separation in bioseparation processes | |
| |
| |
| |
| Physical forms separated in bioseparation | |
| |
| |
| |
| Bioseparation techniques | |
| |
| |
| |
| The RIPP scheme | |
| |
| |
| |
| Example of bioseparation | |
| |
| |
| |
| Current trends in the bioseparation | |
| |
| |
| |
| Properties of biological material | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Size | |
| |
| |
| |
| Molecular weight | |
| |
| |
| |
| Diffusivity | |
| |
| |
| |
| Sedimentation coefficient | |
| |
| |
| |
| Osmotic pressure | |
| |
| |
| |
| Electrostatic charge | |
| |
| |
| |
| Solubility | |
| |
| |
| |
| Partition coefficient | |
| |
| |
| |
| Light absorption | |
| |
| |
| |
| Fluorescence | |
| |
| |
| |
| Mass transfer | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Molecular diffusion in liquid medium | |
| |
| |
| |
| Measurement of diffusivity | |
| |
| |
| |
| Estimation of diffusivity | |
| |
| |
| |
| Diffusion of solutes in dense solid | |
| |
| |
| |
| Diffusion of solutes in porous solid | |
| |
| |
| |
| Convective mass transfer | |
| |
| |
| |
| Experimental determination of mass transfer coefficient | |
| |
| |
| |
| Estimation of mass transfer coefficient | |
| |
| |
| |
| Inter-phase mass transfer | |
| |
| |
| |
| Unsteady state mass transfer | |
| |
| |
| |
| Equilibrium and rate processes | |
| |
| |
| |
| Cell disruption | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Cells | |
| |
| |
| |
| Cell disruption using bead mill | |
| |
| |
| |
| Cell disruption using rotor-stator mill | |
| |
| |
| |
| Cell disruption using French press | |
| |
| |
| |
| Cell disruption using ultrasonic vibrations | |
| |
| |
| |
| Cell disruption using detergents | |
| |
| |
| |
| Cell disruption using enzymes | |
| |
| |
| |
| Cell disruption using organic solvents | |
| |
| |
| |
| Cell disruption by osmotic shock | |
| |
| |
| |
| Precipitation | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Factors utilized for precipitation | |
| |
| |
| |
| Precipitation using organic solvents | |
| |
| |
| |
| Precipitation using anti-chaotropic salts | |
| |
| |
| |
| Mechanism of precipitate formation | |
| |
| |
| |
| Centrifugation | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Laboratory centrifuge | |
| |
| |
| |
| Preparative centrifuge | |
| |
| |
| |
| Ultracentrifugation | |
| |
| |
| |
| Extraction | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Solvent systems | |
| |
| |
| |
| Theory of extraction | |
| |
| |
| |
| Aqueous two-phase extraction | |
| |
| |
| |
| Batch extraction | |
| |
| |
| |
| Single-stage continuous extraction using immiscible solvents | |
| |
| |
| |
| Batch extraction using partially miscible solvents | |
| |
| |
| |
| Cross-current continuous extraction using immiscible solvents | |
| |
| |
| |
| Staged counter-current extraction | |
| |
| |
| |
| Differential extraction | |
| |
| |
| |
| Supercritical fluid extraction | |
| |
| |
| |
| Adsorption | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Adsorbents | |
| |
| |
| |
| Separation mechanisms | |
| |
| |
| |
| Adsorption isotherms | |
| |
| |
| |
| Diffusional limitations in adsorption processes | |
| |
| |
| |
| Batch adsorption | |
| |
| |
| |
| Packed bed adsorption | |
| |
| |
| |
| Other types of adsorption devices | |
| |
| |
| |
| Chromatography | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Chromatography system | |
| |
| |
| |
| Theory of chromatography | |
| |
| |
| |
| Shape and yield of a chromatographic peak | |
| |
| |
| |
| Binary chromatography | |
| |
| |
| |
| Hydrodynamic chromatography | |
| |
| |
| |
| Filtration | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Theory of filtration | |
| |
| |
| |
| Filter medium | |
| |
| |
| |
| Driving force | |
| |
| |
| |
| Constant pressure cake filtration | |
| |
| |
| |
| Constant rate cake filtration | |
| |
| |
| |
| Improvement of filtration efficiency | |
| |
| |
| |
| Mode of operation | |
| |
| |
| |
| Filtration equipment | |
| |
| |
| |
| Membrane based bioseparation | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Classification of membrane processes | |
| |
| |
| |
| Membrane equipment | |
| |
| |
| |
| Ultrafiltration | |
| |
| |
| |
| Microfiltration | |
| |
| |
| |
| Dialysis | |
| |
| |
| |
| Liquid membrane processes | |
| |
| |
| |
| Membrane chromatography | |
| |
| |
| |
| Miscellaneous bioseparation processes | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| Electrophoresis | |
| |
| |
| |
| Affinity ultrafiltration | |
| |
| |
| |
| Field-flow fractionation | |
| |
| |
| Index | |