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| miRNAs Targeting and Targeting miRNAs | |
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| miRNA Biology | |
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| miRNAs Biogenesis | |
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| miRNAs Actions | |
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| miRNA Expression, Mutation and Polymorphism | |
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| miRNA Expression | |
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| miRNA Mutation | |
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| miRNA Polymorphism | |
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| miRNAs and Human Disease | |
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| miRNAs and Developmental Disorders | |
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| miRNAs and Apoptosis | |
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| miRNAs and Cancer | |
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| miRNAs and Cardiovascular Disease | |
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| miRNAs and Neuronal Disease | |
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| miRNAs and Viral Disease | |
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| miRNAs and Metabolic Disorders | |
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| miRNA and Epigenetics | |
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| miRNAs as Therapeutic Targets | |
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| Strategies for Therapeutic Modulation of miRNAs | |
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| Approaches for Therapeutic Modulation of miRNAs | |
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| References | |
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| miRNA Interference Technologies: An Overview | |
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| New Concepts of miRNAi Technologies | |
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| �miRNAi�, A New Concept | |
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| �miRNA as a Regulator of a Cellular Function�, Second New Concept | |
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| �One-Drug, Multiple-Target�, Third New Concept | |
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| �miRNA Seed Family�, Another New Concept | |
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| General Introduction to miRNAi Technologies | |
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| miRNA-Targeting Technologies | |
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| Targeting-miRNA Technologies | |
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| miRNAi Technologies in Basic Research and Drug Design | |
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| References | |
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| Synthetic Canonical miRNA Technology | |
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| Introduction | |
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| Protocols | |
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| Designing SC-miRNAs | |
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| Validating SC-miRNAs | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| miRNA Mimic Technology | |
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| Introduction | |
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| Protocols | |
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| Designing miR-Mimics | |
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| Validating miR-Mimics | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Problems | |
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| References | |
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| Multi-miRNA Hairpins and Multi-miRNA Mimics Technologies | |
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| Introduction | |
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| Protocols | |
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| Construction of Multi-miRNA Hairpins | |
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| Construction of Multi-miRNA Mimics (Chen et al. 2009) | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| miRNA Transgene Technology | |
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| Introduction | |
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| Protocols | |
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| Conventional Transgene Methods | |
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| Artificial Intronic miRNA Methods | |
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| Cre-loxP Knock-in Methods | |
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| Principle of Actions | |
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| Conventional Transgene Methods | |
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| Artificial Intronic miRNA Methods | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| Anti-miRNA Antisense Oligonucleotides Technology | |
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| Introduction | |
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| Protocols | |
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| Designing AMOs | |
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| Modifying AMOs | |
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| Monitoring Delivery Efficiency of AMOs | |
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| Evaluating Functional Effectiveness of AMOs | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| Multiple-Target Anti-miRNA Antisense Oligonucleotides Technology | |
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| Introduction | |
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| Protocols | |
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| Designing MT-AMOs | |
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| Validating MT-AMOs | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| miRNA Sponge Technology | |
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| Introduction | |
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| Protocols | |
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| Designing miRNA Sponges | |
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| Validating miRNA Sponges | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Problems | |
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| References | |
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| miRNA-Masking Antisense Oligonucleotides Technology | |
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| Introduction | |
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| Protocols | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| Sponge miR-Mas Technology | |
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| Introduction | |
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| Protocols | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| Reference | |
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| miRNA Knockout Technology | |
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| Introduction | |
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| Protocols | |
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| Homologous Recombination Methods | |
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| Cre-loxP Methods | |
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| FLP-FRT Deletion Methods | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| Dicer Inactivation Technology | |
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| Introduction | |
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| Protocols | |
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| Neomycin-Expression Cassette Methods (Homologous Recombination) | |
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| Cre-loxP Methods | |
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| Principle of Actions | |
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| Applications | |
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| Advantages and Limitations | |
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| References | |
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| Index | |