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Microcalorimetry of Macromolecules The Physical Basis of Biological Structures

ISBN-10: 111810451X

ISBN-13: 9781118104514

Edition: 2012

Authors: Peter L. Privalov

List price: $185.95
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This is the first textbook on the microcalorimetry of biological molecules. The coverage starts from the basics of thermodynamics (which are unknown for many scientists working in biology), describes evolution of the calorimetric technique, explains how to analyze the calorimetric data, and illustrates these methods with a wide selection of examples. The book provides an essential resource to scientists studying biological molecular structures and the reactions between these structures.
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Book details

List price: $185.95
Copyright year: 2012
Publisher: John Wiley & Sons Canada, Limited
Publication date: 7/31/2012
Binding: Hardcover
Pages: 404
Size: 6.25" wide x 9.25" long x 1.00" tall
Weight: 1.584
Language: English

Thermodynamic Basics of Calorimetry
Energy Units
Heat Capacity
Kirchhoff's Relation
Gibbs Free Energy
Equilibrium Analysis
Two-State Transition
Derivatives of the Equilibrium Constant
Aqueous Solutions
Specificity of Water as a Solvent
Acid-Base Equilibrium
Partial Quantities
Transfer of Solutes into the Aqueous Phase
Hydration Effects
Hydrophobic Force
Hydration of Polar and Nonpolar Groups
Isothermal Reaction Microcalorimetry
The Heat of Mixing Reaction
Mixing of Reagents in Comparable Volumes
Isothermal Titration Microcalorimeter
ITC Experiments
Analysis of the ITC Data
Heat Capacity Calorimetry
Technical Problems
Differential Scanning Microcalorimeter
Determination of the Partial Heat Capacity of Solute Molecules
DSC Experiments
Determination of the Enthalpy of a Temperature-Induced Process
Determination of the van't Hoff Enthalpy
Multimolecular Two-State Transition
Analysis of the Complex Heat Capacity Profile
Correction for Components Refolding
Pressure Perturbation Calorimetry
Heat Effect of Changing Pressure
Pressure Perturbation Experiment
Evolution of the Concept
Chemical Structure
Physical Structure
Restrictions on the Conformation of Polypeptide Chains
Regular Conformations of Polypeptide Chain Proteins
Hierarchy in Protein Structure
Tertiary Structure of Proteins
Quaternary Structure of Proteins
Nucleic Acids
Chemical Structure
Physical Structure
The �-Helix and �-Helical Coiled-Coil
The �-Helix
Calorimetric Studies of �-Helix Unfolding-Refolding
Analysis of the Heat Capacity Function
�-Helical Coiled-Coils
Two-Stranded Coiled-Coils
Three-Stranded Coiled-Coils
�-Helical Coiled-Coil Proteins
Muscle Proteins
Myosin Rod
Leucine Zipper
Discreteness of the Coiled-Coils
Polyproline-II Coiled-Coils
Collagen Superhelix
Hydrogen Bonds in Collagen
Stability of Collagens
Role of Pyrrolidine Rings in Collagen Stabilization
Calorimetric Studies of Collagens
Enthalpy and Entropy of Collagen Melting
Correlation between Thermodynamic and Structural Characteristics of Collagens
Role of Water in Maintaining the Collagen Structure
Thermodynamics of Collagens
Cooperativity of Collagen Unfolding
Factors Responsible for Maintaining the Collagen Coiled-Coil
Flexibility of the Collagen Structure
Biological Aspect of the Collagen Stability Problem
Globular Proteins
Denaturation of Globular Proteins
Proteins at Extremal Conditions
The Main Problems of Protein Denaturation
Heat Denaturation of Proteins
DSC Studies of Protein Denaturation upon Heating
Reversibility of Heat Denaturation
Cooperativity of Denaturation
Heat Capacity of the Native and Denatured States
Functions Specifying Protein Stability
Cold Denaturation
Proteins at Low Temperatures
Experimental Observation of Cold Denaturation
pH-Induced Protein Denaturation
Isothermal pH Titration of Globular Proteins
Denaturant-Induced Protein Unfolding
Use of Denaturants for Estimating Protein Stability
Calorimetric Studies of Protein Unfolding by Denaturants
Urea and GuHCl Interactions with Protein
Unfolded State of Protein
Completeness of Protein Unfolding at Denaturation
Thermodynamic Functions Describing Protein States
Energetic Basis of Protein Structure
Hydration Effects
Proteins in an Aqueous Environment
Hydration of Protein Groups
Hydration of the Folded and Unfolded Protein
Protein in Vacuum
Heat Capacity of Globular Proteins
Enthalpy of Protein Unfolding in Vacuum
Entropy of Protein Unfolding in Vacuum
Back into the Water
Enthalpies of Protein Unfolding in Water
Hydrogen Bonds
Hydrophobic Effect
Balance of Forces Stabilizing and Destabilizing Protein Structure
Protein Folding
Macrostabilities and Microstabilities of Protein Structure
Macrostability of Proteins
Microstability of Proteins
Packing in Protein Interior
Protein Folding Technology
Intermediate States in Protein Folding
Molten Globule Concept
Formation of Protein Structure
Transient State in Protein Folding
Mechanism of Cooperation
Thermodynamic States of Proteins
Multidomain Proteins
Criterion of Cooperativity
Deviations from a Two-State Unfolding-Refolding
Proteins with Internal Homology
Evolution of Multidomain Proteins
Calcium-Binding Proteins
Discreteness in Protein Structure
Macromolecular Complexes
Entropy of Association Reactions
Thermodynamics of Molecular Association
Experimental Verification of the Translational Entropy
Calorimetry of Association Entropy
SSI Dimer Dissociation
Dissociation of the Coiled-Coil
Entropy Cost of Association
Thermodynamics of Molecular Recognition
Calorimetry of Protein Complex Formation
Target Peptide Recognition by Calmodulin
Thermodynamic Analysis of Macromolecular Complexes
Protein-DNA Interaction
Two Approaches
Protein Binding to the DNA Grooves
Binding to the Major Groove of DNA
Binding of the GCN4 bZIP to DNA
Heterodimeric bZIP Interactions with the Asymmetric DNA Site
IRF Transcription Factors
Binding of NF-�B to the PRDII Site
Binding to the Minor Groove of DNA
HMG Boxes
Comparative Analysis of Protein-DNA Complexes
Sequence-Specific versus Non-Sequence-Specific HMGs
Salt-Dependent versus Salt-Independent Components of Binding
Minor versus Major Groove Binding
Concluding Remarks
Assembling Multicomponent Protein-DNA Complex
CC Approach versus PB Theory
Nucleic Acids
Factors Affecting DNA Melting
Melting of Polynucleotides
Calorimetry of Poly(A)-Poly(U)
Short DNA Duplexes
Calorimetry of Short DNA Duplexes
Specificity of the AT-rich DNA Duplexes
DNA Hydration Studied by Pressure Perturbation Calorimetry
The Cost of DNA Bending
Calorimetry of RNA
Calorimetric Studies of Transfer RNAs