Basic Methods in Microscopy Protocols and Concepts from Cells: A Laboratory Manual

Name: Basic Methods in Microscopy Protocols and Concepts from Cells: A Laboratory Manual
Price: 30.89 USD
Availability: InStock
ISBN: 9780879697518

ISBN-10: 0879697512

ISBN-13: 9780879697518

Edition: 2006 (Revised)

Authors: David L. Spector, Robert Goldman

List price: $97.00

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Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual, provide a solid course in the basics of using the microscope in a biology laboratory. Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton…

Book details

List price: $97.00
Copyright year: 2006
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 10/26/2005
Binding: Paperback
Pages: 382
Size: 8.25" wide x 10.50" long x 0.75" tall
Weight: 2.772
Language: English



Preface



Light Microscopy




Confocal Microscopy, Deconvolution, and Structured Illumination Methods




Multiphoton and Multispectral Laser-scanning Microscopy




Preparation of Cells and Tissues for Fluorescence Microscopy




Nonimmunological Fluorescent Labeling of Cellular Structures




Introduction to Immunofluorescence Microscopy




Immunostaining of Microtubules, Microtubule-associated Proteins, and Intermediate Filaments




Immunofluorescence Localization of Actin




Immunofluorescence Localization of Nuclear Proteins




Immunofluorescence Methods for Saccharomyces cerevisiae




Immunofluorescence Methods for Drosophila Tissues




Immunofluorescence Methods for Caenorhabditis elegans




Analyzing DNA Replication: Nonisotopic Labeling




Analyzing RNA Synthesis: Nonisotopic Labeling




Fluorescence In Situ Hybridization to DNA




Whole-Mount Fluorescence In Situ Hybridization to Drosophila Chromosomal DNA




In Situ Hybridization to RNA




Whole-Mount In Situ Detection of RNAs in Vertebrate Embryos and Isolated Organs




Image Production Using Transmission Electron Microscopy




Preparative Methods for Transmission Electron Microscopy




Immunoelectron Microscopy




Microscopy: Lenses, Filters, and Emission/Excitation Spectra



Cautions