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Basic Techniques. Labs | |
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Measurements, Micropipetting, and Sterile Techniques | |
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Basic Culture Techniques | |
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DNA Restriction and Electrophoresis | |
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Dna Modification | |
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Effects of DNA Methylation on Restriction | |
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Plasmid Transformation and Identification | |
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Rapid Colony Transformation of E. coli with Plasmid DNA | |
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Purification and Identification of Plasmid DNA | |
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Plasmid Recombination and Identification | |
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Recombination of Antibiotic Resistance Genes | |
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Transformation of E. coli with Recombinant DNA | |
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Replicating to Identify Mixed E. coli Populations | |
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Purification and Identification of Recombinant Plasmid DNA | |
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Restriction Mapping of Linear and Circular Chromosomes | |
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Restriction Mapping of the l Chromosome | |
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Restriction Mapping of the Plasmid pBR322 | |
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Southern Hybridization | |
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Southern Hybridization of l DNA | |
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Genomic Library Construction and Analysis | |
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Construction of Genomic Library of l DNA | |
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Transformation of E. coli with l Library | |
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Colony Hybridization of l Library | |
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Purification and Identification of l Clones | |
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Cloning by Polymerase Chain Reaction | |
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Amplification and Purification of l DNA Fragment | |
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Recombination of PCR Product and Plasmid pBLU | |
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Transformation of E. coli with PCR/pBLU Product | |
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The Purification and Identification of PCR/pBLU Recombinants | |
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Human Dna Fingerprinting | |
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Detection of an Alu Insertion Polymorphism by Polymerase Chain Reaction | |
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Detection of a VNTR Polymorphism by Polymerase Chain Reaction | |
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Equipment, Supplies, and Reagents | |
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Recipes for Media, Reagents, and Stock Solutions | |
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Restriction Map Data for pAMP, pKAN, pBLU, l and Bacteriophage | |
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Sequence Data for pAMP, pKAN, pBLU, and Bacteriophage l | |