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Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms | |
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Culture Transfer Techniques | |
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Techniques for Isolation of Pure Cultures | |
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Cultural Characteristics of Microorganisms | |
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Microscopy | |
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Microscopic Examination of Stained Cell Preparations | |
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Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount | |
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The Microscopic Measurement of Microorganisms | |
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Darkfield Microscopy | |
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Bacterial Staining | |
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Preparation of Bacterial Smears | |
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Simple Staining | |
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Negative Staining | |
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Gram Stain | |
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Acid-Fast Stain (Ziehl-Neelsen Method) | |
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Differential Staining for Visualization of Bacterial Cell Structures | |
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Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations | |
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Nutritional Requirements: Media for the Routine Cultivation of Bacteria | |
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Use of Differential and Selective Media | |
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Physical Factors: Temperature | |
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Physical Factors: pH of the Extracellular Environment | |
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Physical Factors: Atmospheric Oxygen Requirements | |
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Techniques for the Cultivation of Anaerobic Microorganisms | |
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Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells | |
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The Bacterial Growth Curve | |
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Biochemical Activities of Microorganisms | |
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Extracellular Enzymatic Activities of Microorganisms | |
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Carbohydrate Fermentation | |
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Triple Sugar-Iron Agar Test | |
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IMViC Test | |
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Hydrogen Sulfide Test | |
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Urease Test | |
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Litmus Milk Reactions | |
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Nitrate Reduction Test | |
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Catalase Test | |
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Oxidase Test | |
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Utilization of Amino Acids | |
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Genus Identification of Unknown Bacterial Cultures | |
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The Protozoa | |
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Free-Living Protozoa | |
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Parasitic Protozoa | |
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The Fungi | |
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Cultivation and Morphology of Molds | |
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Yeast Morphology, Cultural Characteristics, and Reproduction | |
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Identification of Unknown Fungi | |
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The Viruses | |
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Cultivation and Enumeration of Bacteriophages | |
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Isolation of Coliphages from Raw Sewage | |
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Physical and Chemical Agents for the Control of Microbial Growth | |
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Physical Agents of Control: Moist Heat | |
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Physical Agents of Control: Environmental Osmotic Pressure | |
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Physical Agents of Control: Electromagnetic Radiation | |
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Chemical Agents of Control: Chemotherapeutic Agents | |
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Determination of Penicillin Activity in the Presence and Absence of Penicillinase | |
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Chemical Agents of Control: Disinfectants and Antiseptics | |
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Microbiology of Food | |
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Microbiological Analysis of Food Products: Bacterial Count | |
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Wine Production | |
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Sauerkraut Production | |
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Microbiology of Water | |
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Standard Qualitative Analysis of Water | |
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Quantitative Analysis of Water: Membrane Filter Method | |
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Microbiology of Soil | |
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Nitrogen Cycle | |
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Microbial Populations in Soil: Enumeration | |
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Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates | |
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Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique | |
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Bacterial Genetics | |
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Enzyme Induction | |
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Bacterial Conjugation | |
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Isolation of a Streptomycin-Resistant Mutant | |
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The Ames Test: A Bacterial Test System for Chemical Carcinogenicity | |
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Biotechnology | |
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Bacterial Transformation | |
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Isolation of Bacterial Plasmids | |
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Restriction Analysis and Electrophoretic Separation | |