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| Preface | |
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| Acknowledgements | |
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| Introduction | |
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| Special chemical requirements of biomolecules | |
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| Factors affecting analyte structure and stability | |
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| pH effects | |
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| Temperature effects | |
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| Effects of solvent polarity | |
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| Buffering systems used in biochemistry | |
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| How does a buffer work? | |
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| Some common buffers | |
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| Additional components often used in buffers | |
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| Quantitation, units and data handling | |
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| Units used in this text | |
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| Quantitation of protein and biological activity | |
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| Objectives of this book Bibliography | |
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| Chromatography | |
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| Principles of chromatography | |
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| The partition coefficient | |
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| Phase systems used in biochemistry | |
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| Liquid chromatography | |
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| Gas chromatography | |
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| Performance parameters used in chromatography | |
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| Retention | |
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| Resolution | |
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| Physical basis of peak broadening | |
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| Plate height equation | |
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| Capacity factor | |
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| Peak symmetry | |
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| Significance of performance criteria in chromatography | |
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| Chromatography equipment | |
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| Outline of standard system used | |
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| Components of a chromatography system | |
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| Stationary phases used | |
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| Elution | |
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| Modes of chromatography | |
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| Ion exchange | |
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| Gel filtration | |
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| Reversed phase | |
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| Hydrophobic interaction | |
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| Affinity | |
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| Immobilised metal affinity chromatography | |
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| Hydroxyapatite | |
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| Open-column chromatography | |
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| Equipment used | |
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| Industrial-scale chromatography of proteins | |
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| High-performance liquid chromatography | |
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| Equipment used | |
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| Stationary phases in HPLC | |
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| Liquid phases in HPLC | |
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| Fast protein liquid chromatography | |
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| Equipment used | |
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| Comparison with HPLC | |
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| Perfusion chromatography | |
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| Theory of perfusion chromatography | |
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| The practice of perfusion chromatography | |
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| Membrane-based chromatography systems | |
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| Theoretical basis | |
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| Applications of membrane-based separations | |
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| Chromatography of a sample protein | |
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| Designing a purification protocol | |
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| Ion exchange chromatography of a sample protein:Glutathione S-transferases | |
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| HPLC of peptides from glutathione S-transferases Bibliography | |
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| Spectroscopic Techniques | |
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| The nature of light | |
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| A brief history of the theories of light | |
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| Wave-particle duality theory of light | |
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| The electromagnetic spectrum | |
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| The Electromagnetic Spectrum | |
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| Transitions in spectroscopy | |
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| Ultraviolet/visible absorption spectroscopy | |
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| Physical basis | |
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| Equipment used in absorption spectroscopy | |
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| Applications of absorption spectroscopy | |
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| Fluorescence spectroscopy | |
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| Physical basis of fluorescence and related phenomena | |
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| Measurement of fluorescence and chemiluminescence | |
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| External quenching of fluorescence | |
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| Uses of fluorescence in binding studies | |
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| Protein-folding studies | |
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| Resonance energy transfer | |
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| Applications of fluorescence in cell biology | |
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| Spectroscopic techniques using plane-polarised light | |
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| Polarised light | |
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| Chirality in biomolecules | |
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| Circular dichroism (CD) | |
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| Equipment used in CD | |
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| CD of biopolymers | |
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| Linear dichroism (LD) | |
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| LD of biomolecules | |
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| Plasmon resonance spectroscopy | |
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| Infrared spectroscopy | |
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| Physical basis of infrared spectroscopy | |
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| Equipment used in infrared spectroscopy | |
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| Uses of infrared spectroscopy in structure d | |