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| Nucleic Acid Structure and Function | |
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| Structure of nucleic acids | |
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| DNA | |
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| RNA | |
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| Hydrophobic interactions | |
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| Different forms of the double helix | |
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| Supercoiling | |
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| Denaturation and hybridization | |
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| Orientation of nucleic acid strands | |
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| Replication of DNA | |
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| Unwinding and rewinding | |
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| Fidelity of replication: proof-reading | |
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| Chromosome replication and cell division | |
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| DNA repair | |
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| Mismatch repair | |
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| Excision repair | |
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| Recombination (post-replication) repair | |
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| SOS repair | |
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| Gene expression | |
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| Transcription | |
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| Translation | |
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| Post-translational events | |
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| Gene organization | |
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| Mutation and Variation | |
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| Variation and evolution | |
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| Fluctuation test | |
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| Directed mutation in bacteria? | |
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| Types of mutations | |
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| Point mutations | |
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| Conditional mutants | |
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| Variation due to larger scale DNA alterations | |
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| Extrachromosomal agents and horizontal gene transfer | |
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| Phenotypes | |
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| Restoration of phenotype | |
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| Reversion and suppression | |
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| Complementation | |
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| Recombination | |
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| Mechanisms of mutation | |
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| Spontaneous mutation | |
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| Chemical mutagens | |
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| Ultraviolet irradiation | |
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| Isolation and identification of mutants | |
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| Mutation and selection | |
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| Replica plating | |
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| Penicillin enrichment | |
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| Isolation of other mutants | |
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| Molecular methods | |
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| Regulation of Gene Expression | |
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| Gene copy number | |
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| Transcriptional control | |
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| Promoters | |
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| Terminators, attenuators and anti-terminators | |
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| Induction and repression: regulatory proteins | |
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| Attenuation: trp operon | |
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| Two-component regulatory systems | |
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| Global regulatory systems | |
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| Feast or famine and the RpoS regulon | |
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| Quorum sensing | |
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| Translational control | |
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| Ribosome binding | |
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| Codon usage | |
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| Stringent response | |
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| Regulatory RNA | |
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| Phase variation | |
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| Genetics of Bacteriophages | |
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| Single-stranded DNA bacteriophages | |
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| oX174 | |
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| M13 | |
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| RNA-containing phages: MS2 | |
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| Double-stranded DNA phages | |
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| Bacteriophage T4 | |
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| Bacteriophage lambda | |
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| Lytic and lysogenic regulation of bacteriophage lambda | |
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| Restriction and modification | |
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| Complementation and recombination | |
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| Why are bacteriophages important? | |
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| Phage typing | |
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| Phage therapy | |
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| Phage display | |
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| Bacterial virulence and phage conversion | |
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| Plasmids | |
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| Some bacterial characteristics are determined by plasmids | |
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| Antibiotic resistance | |
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| Colicins and bacteriocins | |
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| Virulence determinants | |
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| Plasmids in plant-associated bacteria | |
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| Metabolic activities | |
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| Molecular properties of plasmids | |
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| Plasmid replication and control | |
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| Plasmid stability | |
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| Plasmid integrity | |
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| Partitioning | |
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| Differential growth rate | |
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| Methods for studying plasmids | |
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| Associating a plasmid with a phenotype | |
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| Classification of plasmids | |
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| Gene Transfer | |
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| Transformation | |
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| Conjugation | |
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| Mechanism of conjugation | |
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| The F plasmid | |
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| Conjugation in other bacteria | |
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| Transduction | |
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| Specialized transduction | |
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| Recombination | |
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| General (homologous) recombination | |
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| Site-specific and non-homologous (illegitimate) recombination | |
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| Mosaic genes and chromosome plasticity | |
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| Genomic Plasticity: Movable Genes and Phase Variation | |
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| Insertion sequences | |
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| Structure of insertion sequences | |
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| Occurrence of insertion sequences | |
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| Transposons | |
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| Structure of transposons | |
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| Integrons | |
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| Mechanisms of transposition | |
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| Replicative transposition | |
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| Non-replicative (conservative) transposition | |
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| Regulation of transposition | |
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| Activation of genes by transposable elements | |
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| Mu: a transposable bacteriophage | |
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| Conjugative transposons and other transposable elements | |
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| Phase variation | |
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| Variation mediated by simple DNA inversion | |
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| Variation mediated by nested DNA inversion | |
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| Antigenic variation in the gonococcus | |
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| Phase variation by slipped strand mispairing | |
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| Phase variation mediated by differential DNA methylation | |
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| Genetic Modification: Exploiting the Potential of Bacteria | |
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| Strain development | |
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| Generation of variation | |
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| Selection of desired variants | |
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| Overproduction of primary metabolites | |
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| Simple pathways | |
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| Branched pathways | |
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| Overproduction of secondary metabolites | |
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| Gene cloning | |
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| Cutting and joining DNA | |
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| Plasmid vectors | |
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| Transformation | |
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| Bacteriophage lambda vectors | |
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| Cloning larger fragments | |
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| Bacteriophage M13 vectors | |
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| Gene libraries | |
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| Construction of genomic libraries | |
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| Screening a gene library | |
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| Construction of a cDNA library | |
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| Products from cloned genes | |
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| Expression vectors | |
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| Making new genes | |
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| Other bacterial hosts | |
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| Novel vaccines | |
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| Other uses of gene technology | |
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| Genetic Methods for Investigating Bacteria | |
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| Metabolic pathways | |
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| Complementation | |
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| Cross-feeding | |
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| Microbial physiology | |
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| Reporter genes | |
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| Lysogeny | |
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| Cell division | |
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| Motility and chemotaxis | |
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| Cell differentiation | |
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| Bacterial virulence | |
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| Wide range mechanisms of bacterial pathogenesis | |
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| Detection of virulence genes | |
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| Specific mutagenesis | |
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| Gene replacement | |
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| Antisense RNA | |
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| Taxonomy, evolution and epidemiology | |
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| Molecular taxonomy | |
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| Diagnostic use of PCR | |
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| Molecular epidemiology | |
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| Gene Mapping to Genomics | |
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| Gene mapping | |
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| Conjugational analysis | |
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| Co-transformation and co-transduction | |
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| Molecular techniques for gene mapping | |
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| Gene sequencing | |
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| DNA sequence determination | |
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| Genome sequencing | |
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| Comparative genomics | |
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| Bioinformatics | |
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| Physical and genetic maps | |
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| Deletions and insertions | |
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| Transposon mutagenesis | |
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| Gene replacement | |
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| Site-directed mutagenesis | |
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| Analysis of gene expression | |
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| Transcriptional analysis | |
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| Translational analysis | |
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| Systematic analysis of gene function | |
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| Conclusion | |
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| Further Reading | |
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| Abbreviations | |
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| Glossary | |
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| Enzymes | |
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| Genes | |
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| Standard Genetic Code | |
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| Bacterial Species | |
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| Index | |