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Molecular Genetics of Bacteria

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ISBN-10: 0471951110

ISBN-13: 9780471951117

Edition: 2nd 1994

Authors: Jeremy W. Dale

List price: $56.00
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Book details

List price: $56.00
Edition: 2nd
Copyright year: 1994
Publisher: John Wiley & Sons, Incorporated
Publication date: 2/7/1995
Binding: Paperback
Pages: 302
Size: 5.98" wide x 9.02" long x 0.79" tall
Weight: 1.122
Language: English

Nucleic Acid Structure and Function
Structure of nucleic acids
Hydrophobic interactions
Different forms of the double helix
Denaturation and hybridization
Orientation of nucleic acid strands
Replication of DNA
Unwinding and rewinding
Fidelity of replication: proof-reading
Chromosome replication and cell division
DNA repair
Mismatch repair
Excision repair
Recombination (post-replication) repair
SOS repair
Gene expression
Post-translational events
Gene organization
Mutation and Variation
Variation and evolution
Fluctuation test
Directed mutation in bacteria?
Types of mutations
Point mutations
Conditional mutants
Variation due to larger scale DNA alterations
Extrachromosomal agents and horizontal gene transfer
Restoration of phenotype
Reversion and suppression
Mechanisms of mutation
Spontaneous mutation
Chemical mutagens
Ultraviolet irradiation
Isolation and identification of mutants
Mutation and selection
Replica plating
Penicillin enrichment
Isolation of other mutants
Molecular methods
Regulation of Gene Expression
Gene copy number
Transcriptional control
Terminators, attenuators and anti-terminators
Induction and repression: regulatory proteins
Attenuation: trp operon
Two-component regulatory systems
Global regulatory systems
Feast or famine and the RpoS regulon
Quorum sensing
Translational control
Ribosome binding
Codon usage
Stringent response
Regulatory RNA
Phase variation
Genetics of Bacteriophages
Single-stranded DNA bacteriophages
RNA-containing phages: MS2
Double-stranded DNA phages
Bacteriophage T4
Bacteriophage lambda
Lytic and lysogenic regulation of bacteriophage lambda
Restriction and modification
Complementation and recombination
Why are bacteriophages important?
Phage typing
Phage therapy
Phage display
Bacterial virulence and phage conversion
Some bacterial characteristics are determined by plasmids
Antibiotic resistance
Colicins and bacteriocins
Virulence determinants
Plasmids in plant-associated bacteria
Metabolic activities
Molecular properties of plasmids
Plasmid replication and control
Plasmid stability
Plasmid integrity
Differential growth rate
Methods for studying plasmids
Associating a plasmid with a phenotype
Classification of plasmids
Gene Transfer
Mechanism of conjugation
The F plasmid
Conjugation in other bacteria
Specialized transduction
General (homologous) recombination
Site-specific and non-homologous (illegitimate) recombination
Mosaic genes and chromosome plasticity
Genomic Plasticity: Movable Genes and Phase Variation
Insertion sequences
Structure of insertion sequences
Occurrence of insertion sequences
Structure of transposons
Mechanisms of transposition
Replicative transposition
Non-replicative (conservative) transposition
Regulation of transposition
Activation of genes by transposable elements
Mu: a transposable bacteriophage
Conjugative transposons and other transposable elements
Phase variation
Variation mediated by simple DNA inversion
Variation mediated by nested DNA inversion
Antigenic variation in the gonococcus
Phase variation by slipped strand mispairing
Phase variation mediated by differential DNA methylation
Genetic Modification: Exploiting the Potential of Bacteria
Strain development
Generation of variation
Selection of desired variants
Overproduction of primary metabolites
Simple pathways
Branched pathways
Overproduction of secondary metabolites
Gene cloning
Cutting and joining DNA
Plasmid vectors
Bacteriophage lambda vectors
Cloning larger fragments
Bacteriophage M13 vectors
Gene libraries
Construction of genomic libraries
Screening a gene library
Construction of a cDNA library
Products from cloned genes
Expression vectors
Making new genes
Other bacterial hosts
Novel vaccines
Other uses of gene technology
Genetic Methods for Investigating Bacteria
Metabolic pathways
Microbial physiology
Reporter genes
Cell division
Motility and chemotaxis
Cell differentiation
Bacterial virulence
Wide range mechanisms of bacterial pathogenesis
Detection of virulence genes
Specific mutagenesis
Gene replacement
Antisense RNA
Taxonomy, evolution and epidemiology
Molecular taxonomy
Diagnostic use of PCR
Molecular epidemiology
Gene Mapping to Genomics
Gene mapping
Conjugational analysis
Co-transformation and co-transduction
Molecular techniques for gene mapping
Gene sequencing
DNA sequence determination
Genome sequencing
Comparative genomics
Physical and genetic maps
Deletions and insertions
Transposon mutagenesis
Gene replacement
Site-directed mutagenesis
Analysis of gene expression
Transcriptional analysis
Translational analysis
Systematic analysis of gene function
Further Reading
Standard Genetic Code
Bacterial Species