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| Preface to Part I | |
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| Acknowledgment | |
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| Introduction to Part I | |
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| Instrumentation and Developments | |
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| Introduction | |
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| Ionization Techniques for Macromolecules | |
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| Examples on Analytical Solutions Based on FAB-MS | |
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| Electrospray Ionization | |
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| Matrix-Assisted Laser Desorption Ionization | |
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| Ion Detection | |
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| Types of Analyzers | |
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| Hybrid Analyzers | |
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| Tandem Mass Spectrometry | |
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| Current MS Instrumentation in Proteome Analyses | |
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| Current MS-Based Proteomics | |
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| Recent Achievements and Future Challenges | |
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| Concluding Remarks | |
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| References | |
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| Proteomics in Cancer Research | |
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| Introduction | |
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| Pancreatic Ductal Adenocarcinoma | |
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| Proteomic Analysis of Human Breast Carcinoma | |
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| Proteomic Profiling of Chemoresistant Cancer Cells | |
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| Signal Pathway Profiling of Prostate Cancer | |
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| Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding | |
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| Activity-Based Protein Profiling | |
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| Probing Protein Functions Using Chromophore-Assisted Laser Inactivation | |
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| Role of Protein-Tyrosine Kinases | |
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| Concluding Remarks and Future Prospects | |
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| References | |
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| Current Strategies for Protein Quantification | |
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| Introduction | |
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| Global Internal Standard Technology | |
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| Differential In-Gel Electrophoresis | |
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| Quantification of Modified Proteins | |
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| Comments and Considerations | |
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| Other Approaches | |
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| Emerging Role of Microfluidic Devices | |
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| Concluding Remarks | |
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| References | |
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| Proteomics Today: Separation Science at Work | |
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| Conventional Isoelectric Focusing in Gel Matrices and Capillaries and Immobilized Ph Gradients | |
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| Introduction | |
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| Conventional Isoelectric Focusing in Amphoteric Buffers | |
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| Immobilized pH Gradients | |
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| Capillary Isoelectric Focusing | |
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| Separation of Peptides and Proteins by CZE in Isoelectric Buffers | |
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| Conclusions | |
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| References | |
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| Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis | |
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| Introduction | |
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| SDS-Protein Complexes: a Refinement of the Model | |
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| Theoretical Background of Mr Measurement by SDS-PAGE | |
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| Methodology | |
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| Gel Casting and Buffer Systems | |
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| Blotting Procedures | |
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| Conclusions | |
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| References | |
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| Two-Dimensional Maps | |
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| Introduction | |
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| Some Basic Methodology Pertaining to 2D PAGE | |
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| Prefractionation Tools in Proteome Analysis | |
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| Multidimensional Chromatography Coupled to MS | |
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| Protein Chips and Microarrays | |
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| Nondenaturing Protein Maps | |
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| Spot Matching in 2D Gels via Commercial Software | |
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| Conclusions | |
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| References | |
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| Index | |