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Proteomics Today Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology

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ISBN-10: 0471648175

ISBN-13: 9780471648178

Edition: 2005

Authors: Mahmoud H. Hamdan, Pier G. Righetti

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The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well. Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome Explains the hows and whys of using mass spectrometry in proteomic analysis Brings together the latest approaches…    
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Book details

Copyright year: 2005
Publisher: John Wiley & Sons, Incorporated
Publication date: 2/11/2005
Binding: Hardcover
Pages: 448
Size: 6.25" wide x 9.25" long x 1.00" tall
Weight: 1.628
Language: English

Preface to Part I
Introduction to Part I
Instrumentation and Developments
Ionization Techniques for Macromolecules
Examples on Analytical Solutions Based on FAB-MS
Electrospray Ionization
Matrix-Assisted Laser Desorption Ionization
Ion Detection
Types of Analyzers
Hybrid Analyzers
Tandem Mass Spectrometry
Current MS Instrumentation in Proteome Analyses
Current MS-Based Proteomics
Recent Achievements and Future Challenges
Concluding Remarks
Proteomics in Cancer Research
Pancreatic Ductal Adenocarcinoma
Proteomic Analysis of Human Breast Carcinoma
Proteomic Profiling of Chemoresistant Cancer Cells
Signal Pathway Profiling of Prostate Cancer
Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding
Activity-Based Protein Profiling
Probing Protein Functions Using Chromophore-Assisted Laser Inactivation
Role of Protein-Tyrosine Kinases
Concluding Remarks and Future Prospects
Current Strategies for Protein Quantification
Global Internal Standard Technology
Differential In-Gel Electrophoresis
Quantification of Modified Proteins
Comments and Considerations
Other Approaches
Emerging Role of Microfluidic Devices
Concluding Remarks
Proteomics Today: Separation Science at Work
Conventional Isoelectric Focusing in Gel Matrices and Capillaries and Immobilized Ph Gradients
Conventional Isoelectric Focusing in Amphoteric Buffers
Immobilized pH Gradients
Capillary Isoelectric Focusing
Separation of Peptides and Proteins by CZE in Isoelectric Buffers
Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis
SDS-Protein Complexes: a Refinement of the Model
Theoretical Background of Mr Measurement by SDS-PAGE
Gel Casting and Buffer Systems
Blotting Procedures
Two-Dimensional Maps
Some Basic Methodology Pertaining to 2D PAGE
Prefractionation Tools in Proteome Analysis
Multidimensional Chromatography Coupled to MS
Protein Chips and Microarrays
Nondenaturing Protein Maps
Spot Matching in 2D Gels via Commercial Software