Introduction to Biophotonics

ISBN-10: 0471287709

ISBN-13: 9780471287704

Edition: 2003

Authors: Paras N. Prasad

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Description:

Biophotonics involes the use of photonics, the technology of generating and harnessing light and other radiant energy, for biological purposes. This book introduces biophotonics to graduate level students and practicing researchers in the allied disciplines.
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Book details

List price: $173.00
Copyright year: 2003
Publisher: John Wiley & Sons, Incorporated
Publication date: 4/8/2003
Binding: Hardcover
Pages: 594
Size: 6.50" wide x 9.50" long x 1.50" tall
Weight: 2.2
Language: English

Preface
Acknowledgements
Introduction
Biophotonics--A New Frontier
An Invitation to Multidisciplinary Education, Training, and Research
Opportunities for Both Basic Research and Biotechnology Development
Scope of this Book
Fundamentals of Light and Matter
Nature of Light
Dual Character of Light
Propagation of Light as Waves
Coherence of Light
Light as Photon Particles
Optical Activity and Birefringence
Different Light Sources
Quantized States of Matter
Introductory Concepts
Quantized States of Atoms
Quantized States of Molecules: Partitioning of Molecular Energies
Electronic States of a Molecule
Bonding in Organic Molecules
Conjugated Organic Molecules
Vibrational States of a Molecule
Intermolecular Effects
Three-Dimensional Structures and Stereoisomers
Basics of Biology
Introductory Concepts
Cellular Structure
Various Types of Cells
Chemical Building Blocks
Interactions Determining Three-Dimensional Structures of Biopolymers
Other Important Cellular Components
Cellular Processes
Protein Classification and Function
Organization of Cells into Tissues
Types of Tissues and Their Functions
Tumors and Cancers
Fundamentals of Light-Matter Interactions
Interactions Between Light and a Molecule
Nature of Interactions
Einstein's Model of Absorption and Emission
Interaction of Light with a Bulk Matter
Fate of Excited State
Various Types of Spectroscopy
Electronic Absorption Spectroscopy
Electronic Luminescence Spectroscopy
Vibrational Spectroscopy
Spectroscopy Utilizing Optical Activity of Chiral Media
Fluorescence Correlation Spectroscopy (FCS)
Principles of Lasers, Current Laser Technology, and Nonlinear Optics
Principles of Lasers
Lasers: A New Light Source
Principles of Laser Action
Classification of Lasers
Some Important Lasers for Biophotonics
Current Laser Technologies
Quantitative Description of Light: Radiometry
Nonlinear Optical Processes with Intense Laser Beam
Mechanism of Nonlinear Optical Processes
Frequency Conversion by a Second-Order Nonlinear Optical Process
Symmetry Requirement for a Second-Order Process
Frequency Conversion by a Third-Order Nonlinear Optical Process
Multiphoton Absorption
Time-Resolved Studies
Laser Safety
Photobiology
Photobiology--At the Core of Biophotonics
Interaction of Light with Cells
Light Absorption in Cells
Light-Induced Cellular Processes
Photochemistry Induced by Exogenous Photosensitizers
Interaction of Light with Tissues
Photoprocesses in Biopolymers
The Human Eye and Vision
Photosynthesis
In Vivo Photoexcitation
Free-Space Propagation
Optical Fiber Delivery System
Articulated Arm Delivery
Hollow Tube Waveguides
In Vivo Spectroscopy
Optical Biopsy
Single-Molecule Detection
Bioimaging: Principles and Techniques
Bioimaging: An Important Biomedical Tool
An Overview of Optical Imaging
Transmission Microscopy
Simple Microscope
Compound Microscope
Kohler Illumination
Numerical Aperture and Resolution
Optical Aberrations and Different Types of Objectives
Phase Contrast Microscopy
Dark-Field Microscopy
Differential Interference Contrast Microscopy (DIC)
Fluorescence Microscopy
Scanning Microscopy
Inverted and Upright Microscopes
Confocal Microscopy
Multiphoton Microscopy
Optical Coherence Tomography
Total Internal Reflection Fluorescence Microscopy
Near-Field Optical Microscopy
Spectral and Time-Resolved Imaging
Spectral Imaging
Bandpass Filters
Excitation Wavelength Selection
Acousto-Optic Tunable Filters
Localized Spectroscopy
Fluorescence Resonance Energy Transfer (FRET) Imaging
Fluorescence Lifetime Imaging Microscopy (FLIM)
Nonlinear Optical Imaging
Second-Harmonic Microscopy
Third-Harmonic Microscopy
Coherent Anti-Stokes Raman Scattering (CARS) Microscopy
Future Directions of Optical Bioimaging
Multifunctional Imaging
4Pi Imaging
Combination Microscopes
Miniaturized Microscopes
Some Commercial Sources of Imaging Instruments
Bioimaging: Applications
Fluorophores as Bioimaging Probes
Endogenous Fluorophores
Exogenous Fluorophores
Organometallic Complex Fluorophores
Near-IR and IR Fluorophore
Two-Photon Fluorophores
Inorganic Nanoparticles
Green Fluorescent Protein
Imaging of Organelles
Imaging of Microbes
Confocal Microscopy
Near-Field Imaging
Cellular Imaging
Probing Cellular Ionic Environment
Intracellular pH Measurements
Optical Tracking of Drug-Cell Interactions
Imaging of Nucleic Acids
Cellular Interactions Probed by FRET/FLIM Imaging
Tissue Imaging
In Vivo Imaging
Future Directions
Commercially Available Optical Imaging Accessories
Optical Biosensors
Biosensors: An Introduction
Principles of Optical Biosensing
Biorecognition
Optical Transduction
Fluorescence Sensing
Fluorescence Energy Transfer Sensors
Molecular Beacons
Optical Geometries of Biosensing
Support for and Immobilization of Biorecognition Elements
Immobilization
Fiber-Optic Biosensors
Planar Waveguide Biosensors
Evanescent Wave Biosensors
Interferometric Biosensors
Surface Plasmon Resonance Biosensors
Some Recent Novel Sensing Methods
Future Directions
Commercially Available Biosensors
Microarray Technology for Genomics and Proteomics
Microarrays, Tools for Rapid Multiplex Analysis
DNA Microarray Technology
Spotted Arrays
Oligonucleotide Arrays
Other Microarray Technologies
Protein Microarray Technology
Cell Microarray Technology
Tissue Microarray Technology
Some Examples of Application of Microarrays
Future Directions
Companies Producing Microarrays
Flow Cytometry
A Clinical, Biodetection, and Research Tool
Basics of Flow Cytometry
Basic Steps
The Components of a Flow Cytometer
Optical Response
Fluorochromes for Flow Cytometry
Data Manipulation and Presentation
Selected Examples of Applications
Immunophenotyping
DNA Analysis
Future Directions
Commercial Flow Cytometry
Light-Activated Therapy: Photodynamic Therapy
Photodynamic Therapy: Basic Principles
Photosensitizers for Photodynamic Therapy
Porphyrin Derivatives
Chlorins and Bacteriochlorins
Benzoporphyrin Derivatives
5-Aminolaevulinic Acid (ALA)
Texaphyrins
Phthalocyanines and Naphthalocyanines
Cationic Photosensitizers
Dendritic Photosensitizers
Applications of Photodynamic Therapy
Mechanism of Photodynamic Action
Light Irradiation for Photodynamic Therapy
Light Source
Laser Dosimetry
Light Delivery
Two-Photon Photodynamic Therapy
Current Research and Future Directions
Tissue Engineering with Light
Tissue Engineering and Light Activation
Laser Tissue Contouring and Restructuring
Laser Tissue Welding
Laser Tissue Regeneration
Femtolaser Surgery
Future Directions
Laser Tweezers and Laser Scissors
New Biological Tools for Micromanipulation by Light
Principle of Laser Tweezer Action
Design of a Laser Tweezer
Optical Trapping Using Non-Gaussian Beams
Dynamic Holographic Optical Tweezers
Laser Scissors
Laser Pressure Catapulting (LPC)
Laser Capture Microdissection (LCM)
Selected Examples of Applications
Manipulation of Single DNA Molecules
Molecular Motors
Protein-Protein Interactions
Laser Microbeams for Genomics and Proteomics
Laser Manipulation in Plant Biology
Laser Micromanipulation for Reproduction Medicine
Future Directions
Technology of Laser Manipulation
Single Molecule Biofunctions
Commercially Available Laser Microtools
Nanotechnology for Biophotonics: Bionanophotonics
The Interface of Bioscience, Nanotechnology, and Photonics
Nanochemistry
Semiconductor Quantum Dots for Bioimaging
Metallic Nanoparticles and Nanorods for Biosensing
Up-Converting Nanophores
PEBBLE Nanosensors for In Vitro Bioanalysis
Nanoclinics for Optical Diagnostics and Targeted Therapy
Future Directions
Biomaterials for Photonics
Photonics and Biomaterials
Bioderived Materials
Bioinspired Materials
Biotemplates
Bacteria as Biosynthesizers for Photonic Polymers
Future Directions
Index
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