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Cloning, Gene Expression, and Protein Purification Experimental Procedures and Process Rationale

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ISBN-10: 0195132947

ISBN-13: 9780195132946

Edition: 2001

Authors: Charles Hardin, Jennifer Edwards, Andrew Riell, David Presutti, William Miller

List price: $133.95
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Description:

The central purpose of this combination lecture/laboratory manual is to present detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate, and analyse plasmids, to produce fusion proteins in bacteria, and to purify theseproteins based on unique chemical properties or substrate affinities. Electrophoresis, Southern and Western blotting and cominatorial techniques are emphasized. Radioisotopes, fluorophores and solvent effects on…    
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Book details

List price: $133.95
Copyright year: 2001
Publisher: Oxford University Press, Incorporated
Publication date: 3/1/2001
Binding: Hardcover
Pages: 448
Size: 8.25" wide x 10.75" long x 0.75" tall
Weight: 2.2
Language: English

Introductory UnitIntroductory Lecture - Introduction to the Biochemical LaboratoryTheory: Course DescriptionTheory: "Central Dogma of Molecular Biology"Theory: Laboratory SafetyTheory: The Scientific Method: Surviving Recipe MentalityTheory: Proactive TroubleshootingTheory: Introduction to the Biotechnology LaboratoryTheory: Error Analysis and Assay SensitivityTheory: Treatment of Analytical DataTheory: Concentration and Temperature Effects on pKaIntroductory Lab 1 - Basic Biochemical Techniques I: Pipet Calibration and Solution PreparationProcess: PipetsIntroductory Lab 2 - Basic Techniques II: Absorbance Spectroscopy and Protein Concentration DeterminationsProcess: AMP and Tryptophan…