RNA Modification

Name: RNA Modification
Price: 228.25 USD
Availability: InStock
ISBN: 9780123741554

ISBN-10: 0123741556

ISBN-13: 9780123741554

Edition: 425th 2007

Authors: Jonatha M. Gott

List price: $225.00

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Description:

The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in…

Book details

List price: $225.00
Edition: 425th
Copyright year: 2007
Publisher: Elsevier Science & Technology
Publication date: 9/13/2007
Binding: Hardcover
Pages: 480
Size: 5.98" wide x 9.02" long x 0.37" tall
Weight: 1.716




Modified Nucleotides



Identifying modifications in RNA by mass spectrometry



Identification of modified residues in RNAs by reverse transcription-based methods.



tRNA Modifications



Detection of enzymatic activity of transfer RNA modification enzymes using radiolabelled tRNA substrates



In vitro detection of the enzymatic activity of folate-dependent tRNA(U54, C5)-methyltransferase



Probing the intermediacy of covalent RNAenzyme complexes in RNA modification enzymes



Identification and characterization of modification enzymes by biochemical analysis of the proteome



Identification of genes encoding tRNA modification enzymes via comparative genomics



Identification and characterization of archaeal and fungal tRNA methyltransferases



Mass spectrometric identification and characterization of RNA-modifying enzymes



Chaplet column chromatography: isolation of a large set of individual RNAs in a single step.



SNO-mediated Modifications



Biochemical Purification of box H/ACA RNPs involved in pseudouridylation



In Vitro Reconstitution and Affinity Purification of Catalytically Active Archaeal Box C/D sRNP Complexes



Identifying effects of snoRNA-guided modifications on the synthesis and function of the yeast ribosome



The U1 snRNA hairpin II as a RNA affinity tag for selecting snoRNP complexes



A Dedicated Computational Approach for the Identification of Archaeal H/ACA sRNAs



Reconstitution of archaeal H/ACA sRNPs and test of their activity