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Biomedical Applications of Light Scattering

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ISBN-10: 0071598804

ISBN-13: 9780071598804

Edition: 2010

Authors: Adam Wax, Vadim Backman

List price: $245.95
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Book details

List price: $245.95
Copyright year: 2010
Publisher: McGraw-Hill Education
Publication date: 10/13/2009
Binding: Hardcover
Pages: 384
Size: 6.40" wide x 9.30" long x 1.23" tall
Weight: 1.540

Introduction to Light Scattering Models
Classical Light Scattering Models
Introduction to Light Scattering
Structure and Organization of Biological Tissue
Basics of Light Scattering Theory
Approximate Solutions to Light Scattering
Review of Computational Light Scattering Codes
Mie Theory Calculators
T-Matrix Calculations
Discrete Dipole Approximation
Time-Domain Codes
Inverse Light Scattering Analysis
Nonuniqueness Problem
III-Conditioned Problem
Light Scattering from Continuous Random Media
3D Continuous Random Media
Mean Differential Scattering Cross Section
Scattering Coefficient and Related Parameters
Simplifying Approximations
2D Continuous Random Media
Mean Differential Scattering Cross Section
Scattering Coefficient and Related Parameters
1D Continuous Random Media
Generation of Continuous Random Media Samples
Modeling of Light Scattering by Biological Tissues Via Computational Solution of Maxwell's Equations
Overview of FDTD Techniques for Maxwell's Equations
Advantages of FDTD Solution Techniques for Maxwell's Equations
Characteristics of the Yee-Algorithm FDTD Technique
FDTD Modeling Applications
Vertebrate Retinal Rod
Precancerous Cervical Cells
Validation of the Born Approximation in 2D Weakly Scattering Biological Random Media
Sensitivity of Backscattering Signatures to Nanometer-Scale Cellular Changes
Overview of Liu's Fourier-Basis PSTD Technique for Maxwell's Equations
PSTD Modeling Applications
Total Scattering Cross Section of a Round Cluster of 2D Dielectric Cylinders
Enhanced Backscattering of Light by a Large Rectangular Cluster of 2D Dielectric Cylinders
Optical Phase Conjugation for Turbidity Suppression
Multiple Light Scattering in 3D Random Media
Interferometric Synthetic Aperture Microscopy
Physics of Data Acquisition
Compact Forward Model
Rigorous Forward Model
Inverse Scattering Procedure
Numerical Simulations for a Single Scatterer
Alternate Acquisition Geometries
Experimental Implementation and Validation
Phase Stability and Data Acquisition Requirements
Three-Dimensional ISAM of Tissue Phantoms
Cross-Validation of ISAM and OCT
ISAM Processing and Real-Time Implementation
Practical Limitations
Clinical and Biological Applications
Optical Biopsy
Surgical Guidance
Imaging Tumor Development
Conclusions and Future Directions
Application to In Vitro Cell Biology
Light Scattering as a Tool in Cell Biology
Light Scattering Assessments of Mitochondrial Morphology
Light Scattering Assessments of Lysosomal Morphology
Light Scattering Assessments of Nuclear Morphology
Light Scattering Assessments of General Subcellular Structure
Future Perspectives
Light Absorption and Scattering Spectroscopic Microscopies
Absorption and Scattering in Microscopic Applications
Physical Principles and Basic Parameters of Elastic Light Scattering
Light Scattering from Cells and Subcellular Structures
Confocal Light Absorption and Scattering Spectroscopic (CLASS) Microscopy
Applications of CLASS Microscopy
Assessing Bulk Tissue Properties from Scattering Measurements
Light Scattering in Confocal Reflectance Microscopy
The Basic Idea
Theory Mapping (?, ?) to (?s, g)
Experimental Data
Basic Instrument
Monte Carlo Simulations
Current Ongoing Work
Literature Describing Confocal Reflectance Measurements
Tissue Ultrastructure Scattering with Near-Infrared Spectroscopy: Ex Vivo and In Vivo Interpretation
Understanding Light Scattering Measurements in Tissue
Ex Vivo Measurements: Analysis of Scatter Signatures
Microsampling Reflectance Spectroscopy
Phase-Contrast Microscopy
Electron Microscopy: Understanding the Submicroscopic Source of Scatter
Diagnostic Imaging: Approaches for In Vivo Use
Therapeutic Imaging: Surgical Assist
Dynamic Light Scattering Methods
Dynamic Light Scattering and Motility-Contrast Imaging of Living Tissue
Dynamic Light Scattering and Speckle
Single-Mode Scattering
Planar Scattering
Volumetric Scattering
Spatial Homodyne and Heterodyne
Dynamic Scattering
Holographic Optical Coherence Imaging
Fourier-Domain Holography
Digital Holography
Multicellular Tumor Spheroids
Biology in Three Dimensions
Holographic Optical Coherence Imaging of Tumor Spheroids
Subcellular Motility in Tissues
Motility-Contrast Imaging
Conclusions and Prospects
Laser Speckle Contrast Imaging of Blood Flow
Single-Exposure Laser Speckle Contrast Imaging
Applications of LSCI to Brain Imaging
Methodological Details for Imaging CBF Using LSCI
Functional Brain Activation
Multiexposure Laser Speckle Contrast Imaging (MESI)
MESI Theory
MESI Instrument
MESI Measurements in Microfluidics Flow Phantoms
Future Directions
Clinical Applications
Elastic-Scattering Spectroscopy for Optical Biopsy: Probe Designs and Analytical Methods for Clinical Applications
Fiberoptic Probe Designs
Single Optical Fiber Probes
Differential Pathlength Spectroscopy
Angled Probes
Probes Incorporating Full and Half-Ball Lenses
Side-Sensing Probes
Diffusing-Tip Probes
Polarized Probes
Models for the Reflectance Spectra
Methods for Analyzing Reflectance Spectra
A Quantitative Analytical Model Well-Suited to Superficial Tissues
Influence of Blood Vessel Radius
In Vivo Application in a Human Study
Influence of Probe Pressure
Influence of Probe Pressure on Normal Colon Mucosa: A Preliminary Clinical Study
Influence of Probe Pressure on Reflectance Measurements: A Quantitative Animal Study
Temporal Influence of Probe Pressure on Reflectance Measurements: An Animal Study
Differential Pathlength Spectroscopy
Basic Concepts
Main Properties and Features
Basic Mathematical Analysis of Spectra
DPS Measurements In Vivo
Main Features
Additional Spectral Features
Confidence Intervals
Clinical Measurements
Angle-Resolved Low-Coherence Interferometry: Depth-Resolved Light Scattering for Detecting Neoplasia
Early Implementations
Frequency-Domain Implementation
Portable System
Processing of a/LCI Signals
Data Processing for Phantoms
Data Processing for Cell Nuclei
Validation Studies
Polystyrene Microspheres
In Vitro Cell Studies
Tissue Studies
Animal Studies
Human Esophageal Epithelium
Enhanced Backscattering and Low-Coherence Enhanced Backscattering Spectroscopy
Principles of Enhanced Backscattering
Overview and Further Reading
Theory of EBS
Applications of EBS
Low-Coherence Enhanced Backscattering
Enhanced Backscattering of Partially Coherent Light
Observation of Low-Coherence Enhanced Backscattering
Characteristics of LEBS
Theory of LEBS in Tissue
Applications of Low-Coherence Enhanced Backscattering Spectroscopy
Colorectal Cancer
LEBS Detection of Early Cancerous Alterations in Colon Carcinogenesis