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Biophysical Chemistry

ISBN-10: 1849730814

ISBN-13: 9781849730815

Edition: 2nd 2011

Authors: Alan Cooper, David Phillips, E. W. Abel, J. Derek Woollins, Martyn Berry

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Book details

Edition: 2nd
Copyright year: 2011
Publisher: Royal Society of Chemistry, The
Publication date: 2/23/2011
Binding: Paperback
Pages: 244
Size: 7.50" wide x 9.50" long x 0.75" tall
Weight: 1.034
Language: English

Alan Cooper is a Professor of Biophysical Chemistry in the Chemistry Department at Glasgow University, Glasgow, UK. After obtaining a first class honours degree in physics from Manchester University, he gained a diploma for Advanced Studies in Science (with Distinction) on the calculation of dispersion forces and optical properties of helical polynucleotides and a PhD in Biophysics on the thermodynamics of assembly and stability of collagen - both from Manchester University. Since then he has been a SRC Research Fellow at the Laboratory of Molecular Biophysics, Oxford University and a Junior Research Fellow, Wolfson College, Oxford as well as a NIH Research Fellow on the Pathology of Membranes Training Program in the department of Chemistry, Yale University, USA, before his return to Glasgow University as a Lecturer in Physical Chemistry, then Senior Lecturer, Reader and finally Professor. Until 2009, he was the Principal Editor of the Biophysical Chemistry journal and is on the Editorial boards of the European Biophysics Journal, Journal of the Iranian Chemical Society and BMC Biochemistry. He is an external assessor for various European universities and a member of the Molecular Biosciences Foresight Working Party, the ESF Working Party on post-genome biophysical studies and is on the management committee of COST D22 protein-lipid interaction. His research interests include biophysical chemistry, microcalorimetry; thermodynamics of protein folding, misfolding, aggregation and interactions; biomaterials chemistry, protein foams, nematode polyproteins; peptide-antibiotic interactions, and he has also had over 150 full papers published.

David Phillips is Emeritus Professor of Comparative Education at the University of Oxford, UK, and an Emeritus Fellow of St Edmund Hall, Oxford, UK.

Derek Woollings obtained his BSc and PhD at University of East Anglia, UK. He was a lecturer at Imperial College and Professor at the University of Loughborough. He has been Professor at University of St Andrews since 1999 and Director of Research at the School of Chemistry since 2004. Woollins has authored over 400 research papers.

Biological Molecules
Introduction
Proteins and Polypeptides
Polynucleotides
Polysaccharides
Fats, Lipids and Detergents
Water
Foams, Surfactants and Emulsions
Acids, Bases, Buffers and Polyelectrolytes
A Note about Units
Problems
References
Further Reading
Spectroscopy
Electromagnetic Waves and their Interactions
UV/Visible Spectroscopy
Circular Dichroism
Fluorescence
Vibrational Spectroscopy: IR and Raman
NMR (Brief Overview)
Problems
References
Further Reading
Mass Spectrometry
Introduction
Ion Sources
Ionization Methods
Mass Analysers
Detection
Applications of MS
Problems
References
Further Reading
Hydrodynamics
Density and Molecular Volume
Analytical Ultracentrifugation
Sedimentation Equilibrium
Sedimentation Rate
Diffusion and Brownian Motion
Dynamic Light Scattering (DLS)
Viscosity
Problems
References
Further Reading
Thermodynamics and interactions
A Bluffer's Guide to Molecular Thermodynamics
Differential Scanning Calorimetry
Isothermal Titration Calorimetry
Binding Equilibrium
General Methods for Determining Thermodynamic Properties
Thermal Shift Assays
Equilibrium Dialysis
Protein Solubility and Crystallization
Problems
References
Further Reading
Kinetics
Basic Kinetics
Rapid Reaction Techniques
Relaxation Methods
Hydrogen Exchange
Surface Plasmon Resonance
Enzyme Kinetics
Problems
References
Further Reading
Chromatography and Electrophoresis
Chromatography
Electrophoresis
Problems
References
Further Reading
imaging
Waves and Particles
Lenses or No Lenses: Reconstructing the Image
X-ray Diffraction and Protein Crystallography
Fibre Diffraction and Small-angle Scattering
Neutron Diffraction and Scattering
Electron Microscopy
Problems
References
Further Reading
Single Molecules
How Many Molecules can Stand on the Head of a Pin?
Thermodynamic Fluctuations and the Ergodic Hypothesis
Atomic Force Microscopy
Optical Tweezers and Traps
Single Molecule Fluorescence
Problems
References
Further Reading
Answers to Problems
Subject Index