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| Preface to the Sixth Edition | |
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| The Basic Principles of Gene Cloning and DNA Analysis | |
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| Why Gene Cloning and DNA Analysis are Important | |
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| The early development of genetics | |
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| The advent of gene cloning and the polymerase chain reaction | |
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| What is gene cloning? | |
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| What is PCR? | |
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| Why gene cloning and PCR are so important | |
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| How to find your way through this book | |
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| Vectors for Gene Cloning: Plasmids and Bacteriophages | |
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| Plasmids | |
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| Bacteriophages | |
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| Purification of DNA from Living Cells | |
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| Preparation of total cell DNA | |
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| Preparation of plasmid DNA | |
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| Preparation of bacteriophage DNA | |
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| Manipulation of Purified DNA | |
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| The range of DNA manipulative enzymes | |
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| Enzymes for cutting DNA - restriction endonucleases | |
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| Ligation - joining DNA molecules together | |
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| Introduction of DNA into Living Cells | |
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| Transformation - the uptake of DNA by bacterial cells | |
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| Identification of recombinants | |
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| Introduction of phage DNA into bacterial cells | |
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| Identification of recombinant phages | |
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| Introduction of DNA into non-bacterial cells | |
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| Cloning Vectors for E. coli | |
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| Cloning vectors based on E. coli plasmids | |
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| Cloning vectors based on M13 bacteriophage | |
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| Cloning vectors based on l bacteriophage | |
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| � and other high capacity vectors enable genomic libraries to be constructed | |
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| Vectors for other bacteria | |
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| Cloning Vectors for Eukaryotes | |
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| Vectors for yeast and other fungi | |
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| Cloning vectors for higher plants | |
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| Cloning vectors for animals | |
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| How to Obtain a Clone of a Specific Gene | |
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| The problem of selection | |
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| Direct selection | |
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| Identification of a clone from a gene library | |
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| Methods for clone identification | |
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| The Polymerase Chain Reaction | |
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| The polymerase chain reaction in outline | |
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| PCR in more detail | |
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| After the PCR: studying PCR products | |
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| Real time PCR enables the amount of starting material to be quantified | |
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| The Applications of Gene Cloning and DNA Analysis in Research | |
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| Sequencing Genes and Genomes | |
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| The methodology for DNA sequencing | |
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| How to sequence a genome | |
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| Studying Gene Expression and Function | |
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| Studying the RNA transcript of a gene | |
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| Studying the regulation of gene expression | |
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| Identifying and studying the translation product of a cloned gene | |
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| Studying Genomes | |
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| Genome annotation | |
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| Studies of the transcriptome and proteome | |
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| The Applications of Gene Cloning and DNA Analysis in Biotechnology | |
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| Production of Protein from Cloned Genes | |
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| Special vectors for expression of foreign genes in E. coli | |
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| General problems with the production of recombinant protein in E. coli | |
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| Production of recombinant protein by eukaryotic cells | |
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| Gene Cloning and DNA Analysis in Medicine | |
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| Production of recombinant pharmaceuticals | |
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| Identification of genes responsible for human diseases | |
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| Gene therapy | |
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| Gene Cloning and DNA Analysis in Agriculture | |
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| The gene addition approach to plant genetic engineering | |
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| Gene subtraction | |
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| Problems with genetically modified plants | |
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| Gene Cloning and DNA Analysis in Forensic Science and Archaeology | |
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| DNA analysis in the identification of crime suspects | |
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| Studying kinship by DNA profiling | |
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| Sex identification by DNA analysis | |
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| Archaeogenetics - using DNA to study human prehistory | |
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| Glossary | |
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| Index | |