Basic Methods in Protein Purification and Analysis A Laboratory Manual

Name: Basic Methods in Protein Purification and Analysis A Laboratory Manual
Price: 87.5 USD
Availability: InStock
ISBN: 9780879698676

ISBN-10: 0879698675

ISBN-13: 9780879698676

Edition: 2009

Authors: Richard J. Simpson, Peter D. Adams, Erica A. Golemis

List price: $97.00

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Description:

"This new manual is designed for routine, day-to-day use at the bench. By integrating both established in vitro and in vivo molecular techniques with more modern in silico methods, this manual takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. These step-wise methods are combined with troubleshooting advice and guidance on selecting an appropriate experimental strategy, making this new manual a handy yet informative resource for bench…

Book details

List price: $97.00
Copyright year: 2009
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 9/30/2008
Binding: Paperback
Pages: 436
Size: 8.50" wide x 10.75" long x 0.25" tall
Weight: 2.684
Language: English



A Note from the Publisher



Introduction to Protein Purification Strategies




Preparation of Cellular and Subcellular Extracts




Purification Using Gel Electrophoresis



One-dimensional Polyacrylamide Gel Electrophoresis




Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients




Purification Using Column Chromatography



Ion-exchange Chromatography




Size-exclusion Chromatography




Reversed-phase High-performance Liquid Chromatography




Affinity and Immunoaffinity Chromatography




Metal Chelate Affinity Chromatography




Multidimensional Chromatography of Intact Proteins




Characterizing Protein Complexes



Identification of Protein-Protein Interactions by Conventional Column Chromatography




Identification of Associated Proteins by Coimmunoprecipitation




Chromatin Immunoprecipitation of Protein Complexes




Identification of Protein-Protein Interactions with Glutathione-S-Transferase Fusion Proteins




Direct Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation Analysis




In Silico Tools: Analysis for Creating Focused Interaction Networks




Measuring the Concentration of Proteins




Concentrating Solutions of Proteins




Stabilization of Proteins for Storage




Immunoblotting and Staining of Membrane-bound Proteins




General Cautions


Index