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Basic Methods in Protein Purification and Analysis A Laboratory Manual

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ISBN-10: 0879698675

ISBN-13: 9780879698676

Edition: 2009

Authors: Richard J. Simpson, Peter D. Adams, Erica A. Golemis

List price: $97.00
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"This new manual is designed for routine, day-to-day use at the bench. By integrating both established in vitro and in vivo molecular techniques with more modern in silico methods, this manual takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. These step-wise methods are combined with troubleshooting advice and guidance on selecting an appropriate experimental strategy, making this new manual a handy yet informative resource for bench…    
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Book details

List price: $97.00
Copyright year: 2009
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 9/30/2008
Binding: Paperback
Pages: 436
Size: 8.50" wide x 10.75" long x 0.25" tall
Weight: 2.684
Language: English

A Note from the Publisher
Introduction to Protein Purification Strategies
Preparation of Cellular and Subcellular Extracts
Purification Using Gel Electrophoresis
One-dimensional Polyacrylamide Gel Electrophoresis
Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients
Purification Using Column Chromatography
Ion-exchange Chromatography
Size-exclusion Chromatography
Reversed-phase High-performance Liquid Chromatography
Affinity and Immunoaffinity Chromatography
Metal Chelate Affinity Chromatography
Multidimensional Chromatography of Intact Proteins
Characterizing Protein Complexes
Identification of Protein-Protein Interactions by Conventional Column Chromatography
Identification of Associated Proteins by Coimmunoprecipitation
Chromatin Immunoprecipitation of Protein Complexes
Identification of Protein-Protein Interactions with Glutathione-S-Transferase Fusion Proteins
Direct Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation Analysis
In Silico Tools: Analysis for Creating Focused Interaction Networks
Measuring the Concentration of Proteins
Concentrating Solutions of Proteins
Stabilization of Proteins for Storage
Immunoblotting and Staining of Membrane-bound Proteins
General Cautions