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| Safety first! | |
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| Safety in the microbiology laboratory | |
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| Universal precautions | |
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| Culturing bacteria and aseptic techniques | |
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| Preparing culture media | |
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| Sterilization | |
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| The autoclave | |
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| Dry heat | |
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| Aseptic transfer of bacteria | |
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| Adjusting the gas burner | |
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| Preparing bacterial cultures in broth tubes and on agar slants | |
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| Transfer colony from plate to broth and to agar slant | |
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| Isolation of bacteria | |
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| Preparing a pour plate | |
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| Preparing a streak plate | |
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| Preparing a spread plate | |
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| Characteristic features of bacterial growth in broth and on agar | |
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| Maintenance and storage of stock cultures | |
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| Culturing anaerobic bacteria | |
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| Visualizing bacteria | |
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| Effective use and responsible care of the light microscope | |
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| Measuring microscopic cells | |
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| Preparing a hanging-drop slide | |
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| Preparing a bacterial smear | |
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| Preparing a simple stain | |
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| Preparing a Gram stain | |
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| Preparing an acid-fast stain | |
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| Preparing a negative stain | |
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| Preparing a capsule stain | |
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| Preparing an endospore stain | |
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| Enzyme-based tests for the identification of bacteria | |
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| Hydrolytic (digestive) enzymes | |
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| Starch hydrolysis | |
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| Casein hydrolysis | |
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| Gelatin hydrolysis | |
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| Lipid hydrolysis (tributyrin and spirit blue agar) | |
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| DNA hydrolysis | |
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| Utilization of carbohydrates | |
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| Fermentation of carbohydrates (Durham tube) | |
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| Methyl red test (mixed fermentation) | |
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| Voges-Proskauer test (butanediol fermentation) | |
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| Citrate utilization | |
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| Oxidation-fermentation (O-F) glucose test | |
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| Degradation of amino acids | |
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| Indole test | |
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| H2shy;s production | |
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| Phenylalanine deamination | |
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| Decarboxylase test | |
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| Respiration tests | |
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| Catalase test | |
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| Oxidase test | |
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| Nitrate reduction | |
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| Miscellaneous tests | |
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| Triple sugar iron (TSI) test | |
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| Urea hydrolysis | |
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| Litmus milk reactions | |
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| Motility assay | |
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| Coagulase production | |
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| Selective and/or differential media | |
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| Blood agar | |
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| Eosin methylene blue (EMB) agar | |
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| Mannitol-salt agar | |
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| MacConkey agar | |
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| Phenylethyl alcohol (PEA) agar | |
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| Counting microbes | |
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| Direct microscopic counting with the Petroff-Hausser chamber | |
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| Preparing a standard plate count of bacteria | |
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| Using Turbidemetry to estimate cell density | |
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| Plaque assay for determining bacteriophage titer | |
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| Measuring effectiveness of antibacterial chemicals | |
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| Kirby-Bauer method for sensitivity of bacteria to antibacterialmedicines | |
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| Evaluating antibacterial chemicals: The disk-diffusionmethod | |