Cell and Molecular Biology

ISBN-10: 1609135733
ISBN-13: 9781609135737
Edition: 3rd 2012 (Revised)
Authors: Ronald W. Dudek
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Description: High-Yield Cell and Molecular Biology, Third Edition provides the essential information needed for USMLE Step 1 review and course study. It covers current cell and molecular biology techniques and principles with a clinical focuswhat a physician  More...

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Book details

Edition: 3rd
Copyright year: 2012
Publisher: Lippincott Williams & Wilkins
Publication date: 12/21/2010
Binding: Paperback
Pages: 272
Size: 7.00" wide x 10.00" long x 0.25" tall
Weight: 0.682
Language: English

High-Yield Cell and Molecular Biology, Third Edition provides the essential information needed for USMLE Step 1 review and course study. It covers current cell and molecular biology techniques and principles with a clinical focuswhat a physician needs to know to understand, diagnose, and treat human disease. Molecular biology is often taught within various courses such as biochemistry, microbiology, and histology; thus, a consolidated review book in molecular biology is especially helpful in preparing for the USMLE Step 1.

Contents
Preface
Abbreviations
Chromosomal DNA
The Biochemistry of Nucleic Acids
Levels of DNA Packaging
Centromere
Heterochromatin
Euchromatin
Studying Human Chromosomes
Staining of Chromosomes
Chromosome Morphology
DNA Melting Curve
Chromosome Replication
General Features
The Chromosome Replication Process
DNA Topoisomerases
The Telomere
DNA Damage
DNA Repair
Clinical Considerations
Summary of Chromosome Replication Machinery
Meiosis and Genetic Recombination
Meiosis
Genetic Recombination
The Human Nuclear Genome
General Features
Protein-Coding Genes
RNA-Coding Genes
Epigenetic Control
Noncoding DNA
The Human Mitochondrial Genome
General Features
The 13 Protein-Coding Genes
The 24 RNA-Coding Genes
Other Mitochondrial Proteins
Mitochondrial Diseases
Protein Synthesis
General Features
Transcription
Processing the RNA Transcript into mRNA
Translation
Clinical Considerations
Control of Gene Expression
General Features
Mechanism of Gene Expression
The Structure of DNA-Binding Proteins
Other Mechanisms of Gene Expression
The
The
Lac Operon
trp Operon
Mutations of the DNA Sequence
General Features
Silent (Synonymous) Mutations
Non-Silent (Nonsynonymous) Mutations
Loss of Function and Gain of Function Mutations
Other Types of Polymorphisms
Proto-Oncogenes, Oncogenes, and Tumor-Suppressor Genes
Proto-Oncogenes and Oncogenes
Tumor-Suppressor Genes
Hereditary Cancer Syndromes
The Cell Cycle
Mitosis
Control of the Cell Cycle
Molecular Biology of Cancer
The Development of Cancer (Oncogenesis)
The Progression of Cancer
Signal Transduction Pathways
Cell Biology of the Immune System
Neutrophils (Polys, Segs, or PMNs)
Eosinophils
Basophils
Mast Cells
Monocytes
Macrophages (Histiocytes; Antigen-Presenting Cells)
Natural Killer CD
B Lymphocyte
T Lymphocyte
Immune Response to Exogenous Protein Antigens
Immune Response to Endogenous Antigens (Intracellular Virus or Bacteria)
Cytokines
Cell
Molecular Biology of the Immune System
Clonal Selection Theory
The B Lymphocyte (B Cell)
The T Lymphocyte (T Cell)
Clinical Considerations
Disorders of Phagocytic Function
Systemic Autoimmune Disorders
Organ-Specific Autoimmune Disorders
Molecular Biology Techniques
Action of Restriction Enzymes
Electrophoresis
The Enzymatic Method of DNA Sequencing
Southern Blotting and Prenatal Testing for Sickle Cell Anemia
Isolating a Human Gene by DNA Cloning
Construction of cDNA Library
Polymerase Chain Reaction
Producing a Protein from a Cloned Gene
Site-Directed Mutagenesis and Knockout Animals
Northern Blot (mRNA)
Western Blot (Protein)
Human Immunodeficiency Virus (HIV) Structure
Ligase Chain Reaction (LCR)
Flow Cytometry
Identification of Human Disease Genes
General Features
Identification of a Human Disease Gene Through a Chromosome Abnormality
Identification of a Human Disease Gene Through Pure Transcript Mapping
Identification of a Human Disease Gene Through Large Scale DNA Sequencing
Identification of a Human Disease Gene Through Comparison of Human and Mouse Maps
Gene Therapy
Gene Therapy
Ex Vivo and In Vivo Gene Therapy
Integration into Host Cell Chromosomes or as Episomes
Viral Vectors Used in Gene Therapy
Nonviral Vectors Used in Gene Therapy
The Genetic Code
Amino Acids
Chromosomal Locations of Human Genetic Diseases
Figure Credits
Index

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