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Natural Killer Cell Protocols Cellular and Molecular Methods

ISBN-10: 1607613611
ISBN-13: 9781607613619
Edition: 2nd 2010
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Description: As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to  More...

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Book details

Edition: 2nd
Copyright year: 2010
Publisher: Humana Press
Publication date: 12/17/2009
Binding: Hardcover
Pages: 550
Size: 7.25" wide x 10.25" long x 1.50" tall
Weight: 2.530
Language: English

As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of Natural Killer Cell Protocols: Cellular and Molecular Methods brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.

Preface
Contributors
Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells
Subset Analysis of Human and Mouse Mature NK Cells
Assessing Licensing of NK Cells
Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo
Use of Transfected Drosophila S2 Cells to Study NK Cell Activation
Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry
Studying NK Cell/Dendritic Cell Interactions
Analysis of the NK Cell Immunological Synapse
Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry
Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells
A Model System for Studying NK Cell Receptor Signaling
Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction
Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus
Introduction of shRNAs into Primary NK Cells with Lentivirus
Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands
Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation
Identification of NK Cell Receptor Ligands Using a Signaling Reporter System
Determining Ligand Specificity of Ly49 Receptors
Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis
A Simple Method to Measure NK Cell Cytotoxicity In Vivo
Functional Analysis of Human NK Cells by Flow Cytometry
Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry
KIR Genotyping by Multiplex PCR-SSP
Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes
Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus
Studying NK Cell Responses to Ectromelia Virus Infections in Mice
Activation of Human NK Cells by Malaria-Infected Red Blood Cells
Natural Killer Cells in Human Pregnancy
Analysis of Uterine Natural Killer Cells in Mice
Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria
Appendix
Index

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