Laboratory Course in Tissue Engineering

Name: Laboratory Course in Tissue Engineering
Price: 57.75 USD
Availability: InStock
ISBN: 9781439878934

ISBN-10: 1439878935

ISBN-13: 9781439878934

Edition: 2013

Authors: Melissa Kurtis Micou, Dawn Kilkenny

List price: $75.95

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Description:

This book is a hands-on lab manual for tissue engineering (TE) instruction. The manual contains classic TE experiments as well as modern TE techniques, emphasizing the importance of engineering analysis, mathematical modeling, and statistical design of experiments. It includes over 20 stand-alone experiments that provide more than a semester's worth of activities, allowing instructors to customize their courses. The authors present experiments that use equipment commonly found in labs equipped for tissue culture, and they include pre-lab and discussion questions for each experiment.

Book details

List price: $75.95
Copyright year: 2013
Publisher: CRC Press LLC
Publication date: 8/16/2012
Binding: Hardcover
Pages: 304
Size: 6.57" wide x 9.25" long x 0.67" tall
Weight: 1.144
Language: English



Getting Started in the Lab


Lab Safety


Essential Lab Skills for Tissue Engineers


Isolation of Primary Chondrocytes from Bovine Articular Cartilage


Measuring and Modeling Growth of a Cell Population


Purification of a Cell Population Using Magnetic Cell Sorting


Decellularized Matrices for Tissue Engineering


Effect of Plating Density on Cell Adhesion to Varied Culture Matrices


Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds


Cell Patterning Using Microcontact Printing


Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay


Characterizing Matrix Remodeling through Collagen Gel Contraction


Effect of Substrate Stiffness on Cell Differentiation


Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation


Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate


The FahraeusLindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics


Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System


Contribution of Tissue Composition to Bone Material Properties


Technical Communication: Presenting Your Findings



Trypsinizing a Cell Monolayer



Counting Cells with a Hemacytometer



PicoGreenï¿½“DNA Assay



Dimethylmethylene Blue (DMMB) Assay for Sulfated Glycosaminoglycans



Microfluidic Device Design and Fabrication


Index