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Laboratory Course in Tissue Engineering

ISBN-10: 1439878935
ISBN-13: 9781439878934
Edition: 2012
List price: $67.95
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Description: This book is a hands-on lab manual for tissue engineering (TE) instruction. The manual contains classic TE experiments as well as modern TE techniques, emphasizing the importance of engineering analysis, mathematical modeling, and statistical design  More...

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Book details

List price: $67.95
Copyright year: 2012
Publisher: CRC Press LLC
Publication date: 8/16/2012
Binding: Paperback
Pages: 304
Size: 6.50" wide x 9.00" long x 0.75" tall
Weight: 1.100

This book is a hands-on lab manual for tissue engineering (TE) instruction. The manual contains classic TE experiments as well as modern TE techniques, emphasizing the importance of engineering analysis, mathematical modeling, and statistical design of experiments. It includes over 20 stand-alone experiments that provide more than a semester's worth of activities, allowing instructors to customize their courses. The authors present experiments that use equipment commonly found in labs equipped for tissue culture, and they include pre-lab and discussion questions for each experiment.

Getting Started in the Lab
Lab Safety
Essential Lab Skills for Tissue Engineers
Isolation of Primary Chondrocytes from Bovine Articular Cartilage
Measuring and Modeling Growth of a Cell Population
Purification of a Cell Population Using Magnetic Cell Sorting
Decellularized Matrices for Tissue Engineering
Effect of Plating Density on Cell Adhesion to Varied Culture Matrices
Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds
Cell Patterning Using Microcontact Printing
Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay
Characterizing Matrix Remodeling through Collagen Gel Contraction
Effect of Substrate Stiffness on Cell Differentiation
Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation
Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate
The FahraeusLindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics
Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System
Contribution of Tissue Composition to Bone Material Properties
Technical Communication: Presenting Your Findings
Trypsinizing a Cell Monolayer
Counting Cells with a Hemacytometer
PicoGreen�“DNA Assay
Dimethylmethylene Blue (DMMB) Assay for Sulfated Glycosaminoglycans
Microfluidic Device Design and Fabrication
Index

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