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Alzheimer's Disease Methods and Protocols

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ISBN-10: 0896037371

ISBN-13: 9780896037373

Edition: 2000

Authors: Nigel M. Hooper

List price: $109.99
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Description:

A panel of highly skilled investigators describe in detail their state-of-the-art biochemical, cell biological, and molecular biological techniques for studying the molecular basis of Alzheimer's disease. These readily reproducible, step-by-step methods focus on work with the amyloid precursor protein and the amyloidogenic peptide A, but also include-with the recent identification of presenilin proteins-techniques for determining the structure and biological function of these proteins. In addition, there are chapters covering the tau protein and its role in Alzheimer's disease, as well as an introductory discussion of the history of the disease, its genetic basis, and the currently…    
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Book details

List price: $109.99
Copyright year: 2000
Publisher: Humana Press
Publication date: 12/29/1999
Binding: Hardcover
Pages: 408
Size: 6.10" wide x 9.25" long x 0.48" tall
Weight: 3.872

Introduction to Alzheimer's Disease
The Genetics of Alzheimer's Disease
Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimer's Disease
Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPPa)
Quantifying Ab1-40 and Ab1-42 Using Sandwich-ELISA
Electrophoretic Separation and Immunoblotting of Ab1-40 and Ab1-42
Ab-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes
Effects of the b-Amyloid Peptide on Membrane Ion Permeability
Analysis of b-Amyloid Peptide Degradation In Vitro
Posttranslational Modifications of the Amyloid Precursor Protein: Ectodomain Phosphorylation and Sulfation
Posttranslational Modifications of the Amyloid Precursor Protein: Glycosylation
Using an Amyloid Precursor Protein (APP) Reporter to Characterize a-Secretase
Inhibition of a-Secretase by Zinc Metalloproteinase Inhibitors
Development of Neoepitope Antibodies Against the b-Secretase Cleavage Site in the Amyloid Precursor Protein Carol W. Gray and Eric H. Karran. b-Secretase: Tissue Culture Studies of Sequence Specificity, Inhibitors, and Candidate Enzymes
Using g-Secretase Inhibitors to Distiguish the Generation of the Ab Peptides Terminating at Val-40 and Ala-42
Designing Animal Models of Alzheimer's Disease with Amyloid Precursor Protein (APP) Transgenes
Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins
Determining the Transmembrane Topology of the Presenilins
Normal Proteolytic Processing of the Presenilins
Apoptotic Proteolytic Cleavage of the Presenilins by Caspases
The Phosphorylation of Presenilin Proteins
Interaction of the Presenilins with the Amyloid Precursor Protein (APP)
Distribution of Presenilins and Amyloid Precursor Protein (APP) in Detergent-Insoluble Membrane Domains
Characterization and Use of Monoclonal Antibodies to Tau and Paired Helical Filament Tau
Tau Phosphorylation Both In Vitro and in Cells
Transglutaminase-Catalyzed Formation of Alzheimer-Like Insoluble Complexes from Recombinant Tau
Index