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Primer of Genome Science

ISBN-10: 0878932348
ISBN-13: 9780878932344
Edition: 2002
List price: $49.95
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Description: This text provides up-to-date introduction to the field that is suited to advanced undergraduate or early graduate courses. Bioinformatic principles and experimental strategies are explained with the experimental methods.

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Book details

List price: $49.95
Copyright year: 2002
Publisher: Sinauer Associates, Incorporated
Publication date: 12/1/2001
Binding: Paperback
Pages: 347
Size: 6.75" wide x 9.00" long x 0.75" tall
Weight: 1.540
Language: English

This text provides up-to-date introduction to the field that is suited to advanced undergraduate or early graduate courses. Bioinformatic principles and experimental strategies are explained with the experimental methods.

GREG GIBSONis Professor in the School of Integrative Biology at the University of Queensland in Brisbane, Australia, and holds adjunct appointments at North Carolina State University,USAand the Burnham Institute for Medical Research. He earned a Bachelor's Degree in Biology at the University of Sydney, Australiaand a Ph.D. in Cell Biology at the University of Basel, Switzerland(with Walter J. Gehring). Dr. Gibson serves on the editorial boards of several leading journals, and is a Fellow of the American Association for the Advancement of Science. His research is in quantitative genetics and genomics, focusing both on the fruitfly, Drosophila melanogaster, and human variability. SPENCER MUSEis Associate Professor in the Department of Statistics, Bioinformatics Research Center at North Carolina State University, USA. He earned a Bachelor's Degree in Statistics and a Ph.D. in Statistics and Genetics, both at NCSU(the latter with Bruce Weir and Trudy Mackay). Dr. Muse was the recipient of a Sloan Foundation Young Investigator Award (1997-2001). His research is in molecular evolution and bioinformatics.

Preface
Genome Projects: Organization and Objectives
The Core Aims of Genome Science
Mapping Genomes
Genetic Maps
Physical Maps
Cytological Maps
Comparative Genomics
The Human Genome Project
Objectives
The Content of the Human Genome
Internet resources
Animal Genome Projects
Mouse Genome Projects
Other Vertebrate Biomedical Models
Animal Breeding Projects
Invertebrate Model Organisms
Plant Genome Projects
Arabidopsis thaliana
Grasses and Legumes
Other Flowering Plants
Genome Projects
The Minimal Genome
Bacteria and Archaea
Yeast
Parasite Genomics
Summary
Discussion Questions
Literature Cited
Genome Sequencing and Annotation
Automated DNA Sequencing
The Principle of Sanger Sequencing
High-Throughput Sequencing
Reading Sequence Traces
Contig Assembly
Emerging Sequencing Methods
Genome Sequencing
Hierarchical Sequencing
Shotgun Sequencing
Sequence Verification
Genome Annotation
EST Sequencing
Ab initio Gene Discovery
Non-Protein Coding Genes
Structural Features of Genome Sequences
Functional Annotation and Gene Family Clusters
Clustering of Genes by Sequence Similarity
Clusters of Orthologous Genes
Phylogenetic Classification of Genes
Gene Ontology
Summary
Discussion Questions
Web Site Exercises
Literature Cited
Gene Expression and the Transcriptome
Parallel Analysis of Gene Expression: Microarrays
cDNA Microarray Technology
Statistical Analysis of cDNA Microarray Data and Experimental Design
Oligonucleotide Microarray Technology
Microarray Data Mining
SAGE, Microbeads, and Differential Display
Serial Analysis of Gene Expression
Microbead Technology
Differential Display
Single-Gene Analyses
Northern Blots and RNAse Protection
Quantitative PCR
Properties of Transcriptomes
Yeast
Cancer
Model Organisms
Gene Expression Databases
Summary
Discussion Questions
Web Site Exercises
Literature Cited
Proteomics and Functional Genomics
Functional Proteomics
Protein Annotation
Protein Separation and 2D-PAGE
Mass Spectrometry and Protein Identification
Protein Microarrays
Protein Interaction Maps
Structural Proteomics
Objectives of Structural Proteomics
Protein Structure Determination
Protein Structure Prediction and Threading
Functional Genomics
Saturation Forward Genetics
High-Throughput Reverse Genetics
Fine-Structure Genetics
Genetic Fingerprinting
Summary
Discussion Questions
Web Site Exercises
Literature Cited
SNPs and Variation
The Nature of Single Nucleotide Polymorphisms
Classification
Distribution of SNPs
Applications of SNP Technology
Population Genetics
Recombination Mapping
QTL Mapping
Linkage Disequilibrium Mapping
SNP Discovery
Resequencing
Sequence-Free Polymorphism Detection
SNP Genotyping
Low-Technology Methods
Hybridization-Based Methods
Minisequencing Methods
Homogeneous Fluorogenic Dye-Based Methods
Haplotype phasing methods
Summary
Discussion Questions
Web Site Exercises
Literature Cited
Integrative Genomics
Metabolomics
Analysis of Cellular Constituents
Metabolic and Biochemical Databases
In silico Genomics
Metabolic Control Analysis
Systems-Level Modeling of Gene Networks
Summary
Discussion Questions
Literature Cited
Glossary
List of Abbreviations
Index

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