Handbook of Cryo-Preparation Methods for Electron Microscopy

Name: Handbook of Cryo-Preparation Methods for Electron Microscopy
Price: 322.07 USD
Availability: InStock
ISBN: 9780849372278

ISBN-10: 0849372275

ISBN-13: 9780849372278

Edition: 2008

Authors: Annie Cavalier, Bruno M. Humbel, Daniele Spehner

List price: $280.00

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Description:

There has been exponential growth in cryo-electron microscopy methods over the last twenty years. Using an accessible, consistent format to explain the material, Handbook of Cryopreparation Methods for Electron Microscopy presents the most sophisticated methods used in research laboratories. It describes the basic principles of freezing, cryo-ultramicrotomy, embedding at low temperatures and freeze-fracture. The book also explores applications such as electron crystallography, 2D/3D structure, tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols and numerous illustrations to clarify the techniques.

Book details

List price: $280.00
Copyright year: 2008
Publisher: CRC Press LLC
Publication date: 10/2/2008
Binding: Hardcover
Pages: 706
Size: 7.01" wide x 10.28" long x 1.46" tall
Weight: 3.036
Language: English



Introduction


Abbreviations



Vitreous Water




Cryo-Fixation Methods



Slam-Freezing, Metal-Mirror Freezing




Plunge-Freezing (Holey Carbon Method)




Controlled Vitrification




BAL-TEC HPM 010 High-Pressure Freezing Machine




High-Pressure Freezing LEICA EMPACT




Cryo-Electron Microscopy



Frozen-Hydrated Macromolecules for Structural Analysis




Two-Dimensional Crystals




Cryo-Negative Staining




Vitrification of Dynamic Microtubules




CEMOVIS: Cryo-Electron Microscopy of Vitreous Sections




Cryo-Electron Tomography




Low-Temperature Embedding



Freeze-Substitution




Cryo-Fixation, Freeze-Substitution, Rehydration and Tokuyasu Cryo-Sectioning




Freeze-Drying and Embedding of Biological Material




Freeze-Fracture and Metal Shadowing



The Shadow of Hydrated Biological Specimens




Cryo-Fracture of Self-Assembled Systems in Organic Solvent




Analysis



Progressive Lowering of Temperature for Immunolabeling and in situ Hybridization




Cryo-Sectioning According to Tokuyasu




Cryo-Preparation Procedures for Elemental Imaging by SIMS and EFTEM




Correlative Light and Electron Microscopy




SDS-digested Freeze-Fracture Replica Labeling (SDS-FRL)




Immunolabeling of Ultrathin Sections with Enlarged 1 nm Gold or QDots




3-D Electron Tomography of Cells and Organelles



Final Considerations



Glossary


Index