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Principles of Gene Manipulation An Introduction to Genetic Engineering

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ISBN-10: 0632037121

ISBN-13: 9780632037124

Edition: 5th 1994 (Revised)

Authors: R. W. Old, S. B. Primrose

List price: $81.95
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Book details

List price: $81.95
Edition: 5th
Copyright year: 1994
Publisher: John Wiley & Sons, Incorporated
Publication date: 9/27/1994
Binding: Paperback
Pages: 480
Size: 7.44" wide x 9.69" long x 0.35" tall
Weight: 2.486

Introduction to Gene Manipulation
Why do we clone genes?
How do we clone genes?- the basic technique
Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors
Phage and cosmid vectors
Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes
Analyzing DNA nucleotide sequences
Changing genes - macro- and micro-modifications
The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics
Cloning in Organisms other than E. coli
Cloning in bacteria other than E. coli
Cloning in yeast and other microbial eukaryotes
Gene transfer to plants
Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty