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| Introduction | |
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| Safety in the Laboratory | |
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| Unit Introduction | |
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| Creating a Safe Workplace | |
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| Fundamental Principles | |
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| Classroom Activity 1: Performing a Risk Assessment | |
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| Classroom Activity 2: Exploring the CDC and OSHA Websites | |
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| Classroom Activity 3: Responding to Emergencies | |
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| Working Safely with Chemicals | |
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| Fundamental Principles | |
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| Classroom Activity 4: Understanding the Chemicals with which You Work | |
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| Classroom Activity 5: Personal Protection | |
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| Laboratory Exercise 1: Tracking the Spread of Chemical Contamination | |
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| Classroom Activity 6: Analyzing Safety Issues in a Laboratory Procedure | |
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| Working Safely with Biological Hazards | |
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| Fundamental Principles | |
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| Laboratory Exercise 2: Production of Bioaerosols and Factors Affecting Aerosol Production | |
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| Unit Discussion | |
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| Safety Contract, student copy | |
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| Safety Contract, to turn in | |
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| Documentation in the Laboratory | |
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| Unit Introduction | |
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| Classroom Activity 7: Be an Auditor | |
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| Classroom Activity 8: Writing and Following an SOP | |
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| Laboratory Exercise 3: Keeping a Laboratory Notebook | |
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| Unit Discussion | |
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| Metrology in the Laboratory | |
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| Unit Introduction | |
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| Laboratory Exercise 4: Recording Measurements with the Correct Number of Significant Figures | |
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| Classroom Activity 9: Constructing a Simple Balance | |
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| Laboratory Exercise 5:Weight Measurements 1; Good Weighing Practices | |
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| Laboratory Exercise 6: Weight Measurements 2; Performance Verification | |
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| Laboratory Exercise 7: Volume Measurements 1; Proper Use of Volume Measuring Devices | |
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| Laboratory Exercise 8: Volume Measurements 2; Performance Verification of a Micropipette | |
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| Laboratory Exercise 9: Measuring pH with Accuracy and Precision | |
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| Unit Discussion | |
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| Spectrophotometry | |
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| Unit Introduction | |
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| Laboratory Exercise 10: Color and the Absorbance of Light | |
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| Laboratory Exercise 11: Concentration, Absorbance, and Transmittance | |
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| Laboratory Exercise 12: Preparing a Standard Curve with Food Coloring and Using it for Quantitation | |
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| Classroom Activity 10:Beer's Law and Calculating an Absorptivity Constant | |
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| Laboratory Exercise 13: Determination of the Absorptivity Constant for ONP | |
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| Unit Discussion | |
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| Biological Solutions | |
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| Unit Introduction | |
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| Classroom Activity 11: Getting Ready to Prepare Solutions with One Solute: Calculations Classroom Activity 12: Getting Ready to Prepare Solutions with One Solute: Ordering Chemicals | |
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| Laboratory Exercise 14: Preparing Solutions with One Solute | |
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| Laboratory Exercise 15: Preparing Solutions to the Correct Concentration | |
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| Laboratory Exercise 16: Working with Buffers | |
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| Laboratory Exercise 17: Preparing Breaking Buffer | |
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| Laboratory Exercise 18: Preparing TE Buffer | |
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| Laboratory Exercise 19: More Practice Making a Buffer | |
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| Laboratory Exercise 20: Making a Quality Product in a Simulated Company | |
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| Unit Discussion | |
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| Assays | |
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| Unit Introduction | |
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| Laboratory Exercise 21: Two Qualitative Assays | |
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| Laboratory Exercise 22:UV Spectrophotometry of DNA; Quantitative Applications | |
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| Laboratory Exercise 23:UV Spectrophotometry of DNA, RNA, and Proteins; Qualitative Applications | |
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| Laboratory Exercise 24: Using Spectrophotometry for Quality Control; Niacin | |
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| Laboratory Exercise 25: A Quantitative Protein Assay; Learning the Assay | |
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| Laboratory Exercise 26: Exploring Assay Verification | |
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| Laboratory Exercise 27: the Beta-Galactosidase Enzyme Assay | |
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| Laboratory Exercise 28: Comparing the Specific Activity of Two Preparations of Beta-Galactosidase | |
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| Unit Discussion | |
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| Separation Methods | |
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| Unit Introduction | |
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| Classroom Activity 13: Separating Materials Using a Centrifuge, Planning | |
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| Laboratory Exercise 29: Separation of Two Substances Based on Their Differential Affinities for Two Phases | |
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| Laboratory Exercise 30: Separation of Dyes Using Paper Chromatography | |
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| Laboratory Exercise 31: Separating Molecules by Agarose Gel Electrophoresis | |
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| Laboratory Exercise 32: Using Agarose Gel Electrophoresis to Perform an Assay | |
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| Laboratory Exercise 33: Optimizing Agarose Gel Electrophoresis | |
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| Laboratory Exercise 34: Quantification of DNA by Agarose Gel Electrophoresis | |
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| Unit Discussion | |
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| Growing Cells | |
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| Unit Introduction | |
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| Working With Bacterial Cells | |
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| Laboratory Exercise 35: Aseptic Technique on an Open Lab Bench | |
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| Laboratory Exercise 36: Working with Bacteria on an Agar Substrate; Isolating Individual Colonies | |
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| Laboratory Exercise 37: Using a Compound Light Microscope | |
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| Laboratory Exercise 38: Gram Staining | |
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| Laboratory Exercise 39: Making Phosphate Buffered Saline | |
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| Laboratory Exercise 40: the Aerobic Spread Plate Method of Enumerating Colony Forming Units | |
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| Laboratory Exercise 41: Generation of an E. coli Growth Curve | |
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| Working With Mammalian Cells | |
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| Laboratory Exercise 42: Aseptic Technique in a Laminar Flow Hood | |
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| Laboratory Exercise 43: Making Ham's F12 Medium from Dehydrated Powder | |
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| Laboratory Exercise 44: Examining, Photographing, and Feeding CHO (Chinese Hamster Ovary) Cells | |
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| Laboratory Exercise 45: Counting Cells Using a Hemacytometer | |
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| Laboratory Exercise 46: Subculturing CHO Cells | |
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| Laboratory Exercise 47: Preparing a Growth Curve For CHO Cells | |
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| Unit Discussion | |
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| Appendices | |
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| Materials and Supplies that are Assumed to be Present in Your Laboratory | |
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| Calculating Standard Deviation | |
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| Periodic Table of Elements | |