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Free Radicals A Practical Approach

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ISBN-10: 0199635595

ISBN-13: 9780199635597

Edition: 1996

Authors: Neville Punchard, Frank Kelly

List price: $125.00
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With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusive evidence is lacking in many instances, partly due to the difficulties in detecting and measuring free radicals that have lifetimes measured in microseconds. This book outlines the latest techniques for quantifying radicals and their effects, and features detailed protocols, hints, and tips for success, troubleshooting comments, sample data, and key literature citations.
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Book details

List price: $125.00
Copyright year: 1996
Publisher: Oxford University Press
Publication date: 10/10/1996
Binding: Paperback
Pages: 334
Size: 6.14" wide x 9.21" long x 0.71" tall
Weight: 1.276

An architect, Frank S. Kelly is senior vice president and director of planning/programming for the SHW Group, an architectural, planning, and engineering firm focused on architecture for education. SHW's practice extends across much of the country, with offices in Texas, Michigan, and Virginia.Kelly taught design in the School of Architecture at the University of Tennessee and has worked with architectural classes at both Texas A&M and Rice University. With particular interest in the relationship between instruction and facilities, much of his architectural experience has focused on the planning, programming, and design of K-12 schools. He frequently lectures at school conferences related…    

Introduction
Direct and indirect measurements of free radicals by physico-chemical methods
In vitro and in vivo detection of free radical metabolites with electron spin resonance
The application of high-field nuclear magnetic resonance spectroscopy to the detiction of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs
Pulse radiolysis
Direct and indirect measurement of free radicals by other methods
Intrinsic (low-level) chemiluminescence
Visual assessment of oxidative stress by multifunctional digital microfluorography
Salicylic acid and phenylalanine as probes to detect hydroxyl radicals
Measurement of free radical products
Lipids (i): peroxides and other products
Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal
Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipik peroxidation
Proteins (i): determination of carbonyl groups in oxidized proteins
Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA
Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems
Nucleic acids: measurements of products of free radical attack on nucleic acids
Measurement of antioxidants
Glutahione
Glutathione peroxidase: activity and steady-state level of mRNA
Superoxide dismutase
Application of deuterium labelling and gas chromatographt-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids
Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants
Measurement of antioxidant gene expression