Molecular Imaging FRET Microscopy and Spectroscopy

Name: Molecular Imaging FRET Microscopy and Spectroscopy
Price: 41.63 USD
Availability: InStock
ISBN: 9780195177206

ISBN-10: 0195177207

ISBN-13: 9780195177206

Edition: 2005

Authors: Ammasi Periasamy, Richard Day

List price: $131.00

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The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind thevarious FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the…

Book details

List price: $131.00
Copyright year: 2005
Publisher: Elsevier Science & Technology
Publication date: 3/10/2008
Binding: Hardcover
Pages: 336
Size: 7.25" wide x 10.25" long x 1.25" tall
Weight: 2.640
Language: English



Contributors



Proteins and the Flow of Information in Cellular Function




Basics of Fluorescence and FRET




An Introduction to Filters and Mirrors for FRET




FRET Imaging in the Wide-Field Microscope




Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes




Multiphoton FRET Microscopy for Protein Localization in Tissue




FRET Data Analysis: The Algorithm




Photobleaching FRET Microscopy




Single-Molecule FRET




FRET Measurements Using Multispectral Imaging




Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers




Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging




Time-Correlated Single Photon Counting Fluorescence Lifetime Imaging-FRET Microscopy for Protein Localization




Bioluminescence Resonance Energy Transfer: Techniques and Potential




Quantifying Molecular Interactions with Fluorescence Correlation Spectroscopy




Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy



Index