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Plant Microtechnique and Microscopy

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ISBN-10: 0195089561

ISBN-13: 9780195089561

Edition: 1999

Authors: Steven E. Ruzin

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Plant Microtechnique and Microscopy surpases all previously written manuals by providing an in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, flourescence histochemistry, and in situ hybridization, are documented thoroughly. Preceding Chapter One are ten single-page "Quick Start" protocols. Patterned after computer software manuals, these…    
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Book details

Copyright year: 1999
Publisher: Oxford University Press, Incorporated
Publication date: 5/20/1999
Binding: Paperback
Pages: 336
Size: 10.91" wide x 8.39" long x 0.71" tall
Weight: 1.694
Language: English

Introduction
Abbreviations
Quick Start
Microscopy
Chemical Fixation
Tissue Dehydration
Infiltrating and Embedding Tissues
Sectioning and Mounting
Staining
Alternate Methods of Microtomy
Special Methods
Microtechnique Notes and Problem Solving
Histochemistry and Cytochemistry
Localization of Molecular Targets in Tissues
Toxics
Buffers
General Information and Common Calculations
The Mercury Arc Lamp in Fluorescence Microscopy
Manufactures and Vendors
Optics
Bibliography
Index