Plant Microtechnique and Microscopy

ISBN-10: 0195089561
ISBN-13: 9780195089561
Edition: 1999
Authors: Steven E. Ruzin
List price: $77.95
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Description: Plant Microtechnique and Microscopy surpases all previously written manuals by providing an in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background  More...

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Book details

List price: $77.95
Copyright year: 1999
Publisher: Oxford University Press, Incorporated
Publication date: 5/20/1999
Binding: Paperback
Pages: 336
Size: 8.25" wide x 10.75" long x 0.50" tall
Weight: 1.694
Language: English

Plant Microtechnique and Microscopy surpases all previously written manuals by providing an in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, flourescence histochemistry, and in situ hybridization, are documented thoroughly. Preceding Chapter One are ten single-page "Quick Start" protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicites, common calculations, and buffer tables) are provided to make this volume a valuable addition to every biological laboratory. Plant Microtechnique and Microscopy contains a definitive chapter on microscopy and, with diagrams and text, describes the optical principles of techniques such as phase contrast, DIC, confocal and deconvolution wide-field microscopy. Also included is an extensive appendix on optics and its application to the microscope. The book's bibliography of over 650 references is a substantial resource for any student of histology, histological technique, or microscopy.

Introduction
Abbreviations
Quick Start
Microscopy
Chemical Fixation
Tissue Dehydration
Infiltrating and Embedding Tissues
Sectioning and Mounting
Staining
Alternate Methods of Microtomy
Special Methods
Microtechnique Notes and Problem Solving
Histochemistry and Cytochemistry
Localization of Molecular Targets in Tissues
Toxics
Buffers
General Information and Common Calculations
The Mercury Arc Lamp in Fluorescence Microscopy
Manufactures and Vendors
Optics
Bibliography
Index

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