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Techniques for Microbiology A Student Handbook

ISBN-10: 0132240114
ISBN-13: 9780132240116
Edition: 2007
Authors: John M. Lammert
List price: $46.40
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Description: This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental  More...

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Book details

List price: $46.40
Copyright year: 2007
Publisher: Benjamin-Cummings Publishing Company
Publication date: 10/24/2006
Binding: Comb Bound 
Pages: 240
Size: 6.00" wide x 9.00" long x 0.50" tall
Weight: 0.836
Language: English

This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental manipulations and procedures effectively and safely. Demonstrates those techniques that will be used frequently for studying microbes in the laboratory. Has a safety section and frequent safety cautions throughout. Has a convenient, portable 6rdquo; x 9rdquo; trim size, a spiral binding and soft cover, making it ideal for use on the lab bench surface. It is priced inexpensively so that it will be suitable as a supplement to an in-house or commercial manual. Companion to any introductory laboratory whether for biology majors or allied health majors.

Safety first!
Safety in the microbiology laboratory
Universal precautions
Culturing bacteria and aseptic techniques
Preparing culture media
Sterilization
The autoclave
Dry heat
Aseptic transfer of bacteria
Adjusting the gas burner
Preparing bacterial cultures in broth tubes and on agar slants
Transfer colony from plate to broth and to agar slant
Isolation of bacteria
Preparing a pour plate
Preparing a streak plate
Preparing a spread plate
Characteristic features of bacterial growth in broth and on agar
Maintenance and storage of stock cultures
Culturing anaerobic bacteria
Visualizing bacteria
Effective use and responsible care of the light microscope
Measuring microscopic cells
Preparing a hanging-drop slide
Preparing a bacterial smear
Preparing a simple stain
Preparing a Gram stain
Preparing an acid-fast stain
Preparing a negative stain
Preparing a capsule stain
Preparing an endospore stain
Enzyme-based tests for the identification of bacteria
Hydrolytic (digestive) enzymes
Starch hydrolysis
Casein hydrolysis
Gelatin hydrolysis
Lipid hydrolysis (tributyrin and spirit blue agar)
DNA hydrolysis
Utilization of carbohydrates
Fermentation of carbohydrates (Durham tube)
Methyl red test (mixed fermentation)
Voges-Proskauer test (butanediol fermentation)
Citrate utilization
Oxidation-fermentation (O-F) glucose test
Degradation of amino acids
Indole test
H2shy;s production
Phenylalanine deamination
Decarboxylase test
Respiration tests
Catalase test
Oxidase test
Nitrate reduction
Miscellaneous tests
Triple sugar iron (TSI) test
Urea hydrolysis
Litmus milk reactions
Motility assay
Coagulase production
Selective and/or differential media
Blood agar
Eosin methylene blue (EMB) agar
Mannitol-salt agar
MacConkey agar
Phenylethyl alcohol (PEA) agar
Counting microbes
Direct microscopic counting with the Petroff-Hausser chamber
Preparing a standard plate count of bacteria
Using Turbidemetry to estimate cell density
Plaque assay for determining bacteriophage titer
Measuring effectiveness of antibacterial chemicals
Kirby-Bauer method for sensitivity of bacteria to antibacterialmedicines
Evaluating antibacterial chemicals: The disk-diffusionmethod

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