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Molecular and Diagnostic Procedures in Mycoplasmology Diagnostic Procedures

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ISBN-10: 0125838069

ISBN-13: 9780125838061

Edition: 2nd 1996

Authors: Shimuel Razin, Joseph G. Tully

List price: $129.95
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Description:

This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Key Features * Diagnostic genetic probes * Immunological tools * Antibiotic sensitivity testing * Diagnosis of specific diseases * Experimental infections * Diagnosis of mycoplasma infections of cell cultures
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Book details

List price: $129.95
Edition: 2nd
Copyright year: 1996
Publisher: Elsevier Science & Technology Books
Publication date: 2/13/1996
Binding: Hardcover
Pages: 466
Size: 6.50" wide x 9.50" long x 1.00" tall
Weight: 1.782
Language: English

Mollicute-Host Interrelationships: Current Concepts and Diagnostic Implications
Diagnostic Genetic Probes
Introductory Remarks
Oligonucleotide Probes Complementary to 16S rRNA
Cloned Genomics DNA Fragments as Probes
PCR: Selection of Target Sequences
PCR: Preparation of DNA from Clinical Specimens
PCR: Amplified and Identification of Products
PCR: Application of Nested PCR to Detection of Mycoplasmas
PCR: Random Amplified Polymorphic DNA Fingerprinting
Immunological Tools
Introductory Remarks
ELISA in Small Animals, Rodents, and Birds
ELISA in Large Animals
ELISA in Human Urogenital Infections and AIDS
ELISA in Respiratory Infections of Humans
Monoclonal Antibodies as Diagnostic Tools
Microimmunofluorescence
Immunoblots and Immunobinding
Differentiation of Mycloplasma genitalium from Mycoplasma pneumoniae by Immunofluoresence
Antibiotic Sensitivity Testing
Introductory Remarks
Problems and Opportunities in Susceptibility Testing of Mollicutes
Determination of Minimal Inhibitory Concentration
Cidal Activity Testing
Diagnosis of Specific Diseases
Introductory Remarks
Laboratory Diagnosis of Mycoplasma pneumoniae Infections
Diagnosisof Sexually Transmitted Diseases
Diagnosis of Neonatal Infections
Mycoplasmas in AIDS Patients
Mycoplasma Infections of Cattle
Mycoplasma Infections of Goats and Sheep
Mycoplasma Infections of Swine
Mycloplasma Infections of Poultry
Diagnosis of Spiroplasma Infections in Plants and Insects
Detection of Phytoplasma Infections in Plants
Identification of Mollicutes from Insects
Experimental Infections
Introductory Remarks
Experimental Mycoplasmal Respiratory Infections in Rodents
Urogenital Infections in Rodents
Experimental Models of Arthritis
Experimental Infections in Poultry
Experimental Infections of Swine
Experimental Infections in Cattle
Experimental Infections of Plants by Spiroplasmas
Experimental Phytoplasma Infections in Plants and Insects
Mycoplasmas and in Vitro Infections of Cell Cultures with HIV
Diagnosis of Mycoplasma Infections of Cell Cultures
Introductory Remarks
Isolation of Mycoplasmas from Cell Culture by Axenic Cultivation Techniques
Detection o