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| Overview | |
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| Laboratory and Evolutionary History of Tetrahymena thermophila | |
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| Cell Biology of Tetrahymena thermophila | |
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| Tetrahymena Genetics: Two Nuclei are Better Than One | |
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| Methods | |
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| Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance | |
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| Long-term Storage | |
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| Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants | |
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| Methods for Genetic Analysis | |
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| Isolation of Micronuclear and Macronuclear DNA | |
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| Genetically Sorting a Collection of Tetrahymena Mutants | |
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| Genetically Mapping New Mutants and Cloned Genes | |
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| Selection of Motility Mutants | |
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| Nuclear and Cytoskeletal Fluorescence Microscopy Techniques | |
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| Nuclear and Cortical Histology for Brightfield Microscopy | |
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| Fixation of Tetrahymena Cells for Electron Microscopy | |
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| Immunoelectron Microscopy of Tetrahymena | |
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| Cell Biology | |
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| Regulated Protein Secretion in Tetrahymena thermophila | |
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| Electrophysiology of Tetrahymena | |
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| Isolation and Characterization of in Vivo Modified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase | |
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| Tetrahymena Telomerase Activity, Purification, and Reconstruction | |
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| Studying the Telomerase RNA in Tetrahymena | |
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| Isolation and Characterization of 22S Outer Arm Dynein from Tetrahymena Cilia | |
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| Preparation of Cytoskeletal Fractions from Tetrahymena thermophila | |
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| Immunoprecipitation Procedures | |
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| Tetrahymena Calcium-Binding Proteins, TCBP-25 and TCBP-23 | |
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| Manipulating Genes | |
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| Microinjection of Tetrahymena thermophila | |
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| Transient and Stable DNA Transformation of Tetrahymena thermophila by Electroporation | |
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| Biolistic Transformation of Macro- and Micronuclei | |
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| Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in Tetrahymena | |
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| Creation and Use of Antisense Ribosomes in Tetrahymena thermophila | |
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| Protein Tagging in Tetrahymena | |
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| Genetic Nomenclature Rules for Tetrahymena thermophila | |
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| Codon Usage in Tetrahymena thermophila | |
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| Chapter References | |
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| Index | |
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| Volumes in Series | |