Plant Tissue Culture Techniques and Experiments

ISBN-10: 0124159206
ISBN-13: 9780124159204
Edition: 3rd 2012
Authors: Roberta H. Smith
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Description: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media  More...

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Book details

List price: $105.95
Edition: 3rd
Copyright year: 2012
Publisher: Elsevier Science & Technology Books
Publication date: 7/20/2012
Binding: Paperback
Pages: 208
Size: 6.00" wide x 9.00" long x 0.25" tall
Weight: 0.748
Language: English

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature  

Plant Tissue Culture: Techniques and Experiments
Third Edition
Table of Contents
Preface
Acknowledgments
Scheduling and Interrelationships of Exercises
History of Plant Cell Culture
Setup of a Tissue Culture Laboratory
Media Components and Preparation
Explant Preparation
Contamination
Callus Induction
Regeneration and Morphogenesis
Woody Shrubs and Trees
Haploid Plants from Anther Culture
Embryo Rescue
Meristem Culture for Virus-Free Plants
In Vitro Propagation for Commercial Production of Ornamentals
Protoplast Isolation and Fusion
Agrobacterium-Mediated Transformation of Plants
Useful Measurements
Review of Solution Preparation
List of Suppliers
Common Plant Tissue Culture Terms

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