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    Plant Tissue Culture Techniques and Experiments

    ISBN-10: 0124159206
    ISBN-13: 9780124159204
    Edition: 3rd 2012
    Author(s): Roberta H. Smith
    Description: Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media  More...
    List price: $105.95
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    List Price: $105.95
    Edition: 3rd
    Copyright Year: 2012
    Publisher: Elsevier Science & Technology Books
    Publication Date: 7/20/2012
    Binding: Paperback
    Pages: 208
    Size: 6.00" wide x 9.00" long x 0.25" tall
    Weight: 0.748
    Language: English

    Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature  

    Plant Tissue Culture: Techniques and Experiments
    Third Edition
    Table of Contents
    Preface
    Acknowledgments
    Scheduling and Interrelationships of Exercises
    History of Plant Cell Culture
    Setup of a Tissue Culture Laboratory
    Media Components and Preparation
    Explant Preparation
    Contamination
    Callus Induction
    Regeneration and Morphogenesis
    Woody Shrubs and Trees
    Haploid Plants from Anther Culture
    Embryo Rescue
    Meristem Culture for Virus-Free Plants
    In Vitro Propagation for Commercial Production of Ornamentals
    Protoplast Isolation and Fusion
    Agrobacterium-Mediated Transformation of Plants
    Useful Measurements
    Review of Solution Preparation
    List of Suppliers
    Common Plant Tissue Culture Terms

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