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Preface | |
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Introduction | |
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Confocal and Interferometric Microscopy | |
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The Standard Optical Microscope | |
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Principle of Operation | |
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The Point Spread Function | |
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Coherent and Incoherent Illumination | |
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The Coherent Transfer Function, Line Spread Function, and Spatial Frequencies | |
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The Optical Transfer Function | |
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The Rayleigh and Sparrow Two-Point Definitions | |
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Brightness of the Image | |
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Imaging Techniques with the Standard Optical Microscope | |
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The Confocal Microscope | |
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Principle of Operation | |
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Scanning | |
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Depth Response | |
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The Point Spread Function and Two-Point Resolution | |
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History of the CSOM | |
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Optical Interference Microscopes | |
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Principle of Operation | |
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Signal Processing Techniques | |
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Depth and Transverse Resolution | |
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Comparison of Scanning Optical Microscopes with Other Types of Scanning Microscopes | |
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References | |
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Instruments | |
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Introduction | |
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The Confocal Scanning Laser Microscope | |
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The Illumination Source | |
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The Objective Lens | |
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The Scanning Stage | |
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The Intermediate Optics | |
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The Pinhole | |
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The Detector and Electronics | |
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Beam Scanning Techniques | |
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Commercial Examples | |
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Fiber-Optic Scanning Microscopes | |
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Nipkow Disk Scanning Microscopes | |
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One-Sided and Two-Sided Designs | |
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The Nipkow Disk | |
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Illumination of the Disk | |
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The Tilted Disk and Optical Isolator | |
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The Field Lens, Tube Lens, and Objective Lens | |
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The Imaging Path | |
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Commercial Examples | |
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Slit Microscopes | |
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Ophthalmologic Slit Microscopes | |
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Bilateral Scanning Slit Microscopes | |
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Hybrid Slit Microscopes | |
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Confocal Transmission Microscopes | |
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Alternative Imaging Configurations | |
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Interference Microscopes | |
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Interference CSOMs | |
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The Michelson Interference Microscope | |
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The Linnik Interference Microscope | |
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The Mirau Interference Microscope | |
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The Tolanski Interference Microscope | |
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Near-Field Microscopy | |
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The Near-Field Scanning Optical Microscope | |
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Applications of the NSOM | |
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The Solid Immersion Microscope | |
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Conclusion | |
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References | |
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Depth and Transverse Resolution | |
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Introduction | |
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Depth Response of the Confocal Microscope with Infinitesimal Pinholes and Slits | |
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Scalar Theory for a Plane Reflector | |
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Scalar Theory for Depth Response of a Point Reflector | |
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Scalar Theory for Fluorescent Reflectors | |
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Scalar Theory for Confocal Slit Microscopes | |
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The Effect of Sample and Lens Aberrations on the Depth Response | |
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Depth Response of the Confocal Microscope with Finite-Sized Pinholes | |
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Approximate Theory for Optimum Pinhole Size | |
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Approximate Theory for the Range Resolution vs. Pinhole Size | |
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Exact Theory for the Range Resolution vs. Pinhole Size | |
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Transverse Response of the Confocal Microscope | |
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Transverse Response for Infinitesimal Pinholes | |
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Two-Point Resolution | |
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Edge and Line Response | |
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The Effect of Finite Pinhole Size on the Transverse Resolution | |
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Depth and Transverse Resolution of the Interferometric Microscope | |
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Scalar Theory for the Depth Response with a Plane Reflector | |
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Transverse Resolution | |
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The Effect of the Thin-Film Beamsplitter and Mirror Support of the MCM on Signal Levels, Range, and Transverse Resolution | |
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The Near-Field Scanning Optical Microscope (NSOM) | |
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Attenuation in a Tapered Rod or Fiber | |
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The Fields outside the Pinhole | |
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The Solid Immersion Microscope (SIM) | |
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The Transverse and Longitudinal Magnifications of the SIL | |
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The Depth Response of the SIM | |
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The Transverse Response of the SIM | |
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Conclusion | |
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References | |
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Phase Imaging | |
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Introduction | |
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Phase-Contrast Imaging in Conventional Microscopes | |
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Phase-Contrast Imaging in the CSOM | |
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Phase Imaging with an Interferometer | |
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Electro-optic Phase Imaging | |
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The ac Zernike Technique | |
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Acousto-optic Phase Imaging | |
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Differential Interference Contrast Imaging | |
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The Basic Theory of Nomarski Imaging | |
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Imaging Modes of a DIC Microscope | |
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Polarization-Shifted DIC Imaging | |
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Split Detector DIC Imaging | |
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Differential Probe Beam DIC Imaging | |
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Differential Imaging with an AO Modulator | |
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Differential Imaging with an Optical Fiber CSOM | |
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Phase Imaging with an Interference Microscope | |
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The Integrating Bucket Technique | |
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The Fourier Transform Technique | |
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Conclusion | |
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References | |
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Applications | |
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Introduction | |
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Semiconductor Metrology | |
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Microlithography Measurements | |
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Precision, Linearity, and Accuracy in Semiconductor Metrology | |
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Critical Dimension Measurements | |
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Experimental Results | |
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Polarization-Enhanced Imaging of Dense Arrays | |
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Calibration | |
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Overlay Misregistration Measurements | |
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Film Thickness Measurements | |
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CARIS and VAMFO | |
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Film Thickness Measurements with the Mirau Interference Microscope | |
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Biological Imaging | |
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Brightfield and Phase Imaging | |
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Fluorescence Imaging | |
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Two-Wavelength and Two-Photon Fluorescence Imaging | |
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Conclusion | |
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References | |
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Vector Field Theory for Depth and Transverse Resolution of a CSOM | |
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The Depth Response | |
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Transverse Response | |
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References | |
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Index | |