| |
| |
Isolation of Cilia and Flagella | |
| |
| |
Flagellar Amputation and Regeneration in Chlamydomonas | |
| |
| |
Large-Scale Isolation of Chlamydomonas Flagella | |
| |
| |
Isolation of Cilia from Tetrahymena thermophila | |
| |
| |
Preparation of Cilia and Subciliary Fractions from Paramecium | |
| |
| |
Isolation of Euglena Flagella | |
| |
| |
Isolation of Ram Sperm Flagella | |
| |
| |
Isolation of Molluscan Gill Cilia, Sperm Flagella, and Axonemes | |
| |
| |
Isolation of Flagella and Their Membranes from Sea Urchin Spermatozoa | |
| |
| |
Isolation of Fish Sperm Flagella | |
| |
| |
Culture and Isolation of Ciliated Epithelial Cells and Cilia | |
| |
| |
Ciliogenesis of Rat Tracheal Epithelial Cells in Vitro | |
| |
| |
Preparation of Explant and Organ Cultures and Single Cells from Airway Epithelium | |
| |
| |
Induction of Ciliogenesis in Oviduct Epithelium | |
| |
| |
Preparation of Teleost Rod Inner and Outer Segments | |
| |
| |
Isolation of Respiratory Cilia. General Methods | |
| |
| |
Electrophoretic Separation of Dynein Heavy Chains | |
| |
| |
Two-Dimensional Separation of Axonemal Proteins | |
| |
| |
Analysis of Flagellar Calcium-Binding Proteins | |
| |
| |
Phosphorylation of Chlamydomonas Flagellar Proteins | |
| |
| |
In Situ Glycosylation of Flagellar Lipids | |
| |
| |
Detection of Flagellar Protein Kinases on Polyvinylidene Fluoride Membranes Following Sodium Dodecyl Sulfate(Polyacrylamide Gel Electrophoresis | |
| |
| |
Measurement of ATPase Activity Using [gamma- 32 P]ATP | |
| |
| |
Nonradioactive Method for ATPase Assays | |
| |
| |
Structure | |
| |
| |
Immunoelectron Microscopy | |
| |
| |
Immunofluorescence Microscopy of Cilia and Flagella | |
| |
| |
Scanning Transmission Electron Microscopic Analysis of the Isolated Dynein ATPase | |
| |
| |
High-Resolution Negative Staining of the Isolated Dynein ATPase | |
| |
| |
Computer-Assisted Analysis of FlagellarStructure | |
| |
| |
Preparation of Cilia and Flagella for Thin-Section Transmission Electron Microscope Analysis | |
| |
| |
Fixation of Mammalian Spermatozoa for Electron Microscopy. Motors and Motion Analysis | |
| |
| |
Reactivation of Chlamydomonas Cell Models | |
| |
| |
Reactivation of Extracted Paramecium Models | |
| |
| |
Isolation and Reactivation of Newt Lung Respiratory Cilia | |
| |
| |
Reactivation of Motility of Demembranated Sea Urchin Sperm Flagella | |
| |
| |
High-Speed Video Microscopy of Flagella and Cilia | |
| |
| |
Micromanipulation of Sperm and Other Ciliated or Flagellated Single Cells | |
| |
| |
Preparationand Reactivation of Demembranated | |
| |
| |
Cytosol-Free Ram Spermatozoa | |
| |
| |
Microscopic Assays of Flagellar Dynein Activity | |
| |
| |
High-Resolution Imaging of Flagella | |
| |
| |
Flagellar Surface Motility: Gliding and Microsphere Movements | |
| |
| |
Assay of Chlamydomonas Phototaxis | |
| |
| |
Quantification of Ciliary Beat Frequency and Metachrony by High-Speed Digital Video. Cytoskeletal Preparations | |
| |
| |
Isolation and Fractionation of the Tetrahymena Cytoskeleton and Oral Apparatus | |
| |
| |
Release of the Cytosk | |