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Methods in Cell Biology Vol. 47 : Cilia and Flagella

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ISBN-10: 0122097106

ISBN-13: 9780122097102

Edition: 1995

Authors: Leslie Wilson, William Dentler, George Witman, Paul T. Matsudaira

List price: $63.00
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Description:

Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls ofthe procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of"classic"and innovative techniques essential to the study of cilia and flagella. A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced…    
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Book details

List price: $63.00
Copyright year: 1995
Publisher: Elsevier Science & Technology Books
Publication date: 7/25/1995
Binding: Paperback
Pages: 603
Size: 8.25" wide x 9.50" long x 1.00" tall
Weight: 2.838
Language: English

Isolation of Cilia and Flagella
Flagellar Amputation and Regeneration in Chlamydomonas
Large-Scale Isolation of Chlamydomonas Flagella
Isolation of Cilia from Tetrahymena thermophila
Preparation of Cilia and Subciliary Fractions from Paramecium
Isolation of Euglena Flagella
Isolation of Ram Sperm Flagella
Isolation of Molluscan Gill Cilia, Sperm Flagella, and Axonemes
Isolation of Flagella and Their Membranes from Sea Urchin Spermatozoa
Isolation of Fish Sperm Flagella
Culture and Isolation of Ciliated Epithelial Cells and Cilia
Ciliogenesis of Rat Tracheal Epithelial Cells in Vitro
Preparation of Explant and Organ Cultures and Single Cells from Airway Epithelium
Induction of Ciliogenesis in Oviduct Epithelium
Preparation of Teleost Rod Inner and Outer Segments
Isolation of Respiratory Cilia. General Methods
Electrophoretic Separation of Dynein Heavy Chains
Two-Dimensional Separation of Axonemal Proteins
Analysis of Flagellar Calcium-Binding Proteins
Phosphorylation of Chlamydomonas Flagellar Proteins
In Situ Glycosylation of Flagellar Lipids
Detection of Flagellar Protein Kinases on Polyvinylidene Fluoride Membranes Following Sodium Dodecyl Sulfate(Polyacrylamide Gel Electrophoresis
Measurement of ATPase Activity Using [gamma- 32 P]ATP
Nonradioactive Method for ATPase Assays
Structure
Immunoelectron Microscopy
Immunofluorescence Microscopy of Cilia and Flagella
Scanning Transmission Electron Microscopic Analysis of the Isolated Dynein ATPase
High-Resolution Negative Staining of the Isolated Dynein ATPase
Computer-Assisted Analysis of FlagellarStructure
Preparation of Cilia and Flagella for Thin-Section Transmission Electron Microscope Analysis
Fixation of Mammalian Spermatozoa for Electron Microscopy. Motors and Motion Analysis
Reactivation of Chlamydomonas Cell Models
Reactivation of Extracted Paramecium Models
Isolation and Reactivation of Newt Lung Respiratory Cilia
Reactivation of Motility of Demembranated Sea Urchin Sperm Flagella
High-Speed Video Microscopy of Flagella and Cilia
Micromanipulation of Sperm and Other Ciliated or Flagellated Single Cells
Preparationand Reactivation of Demembranated
Cytosol-Free Ram Spermatozoa
Microscopic Assays of Flagellar Dynein Activity
High-Resolution Imaging of Flagella
Flagellar Surface Motility: Gliding and Microsphere Movements
Assay of Chlamydomonas Phototaxis
Quantification of Ciliary Beat Frequency and Metachrony by High-Speed Digital Video. Cytoskeletal Preparations
Isolation and Fractionation of the Tetrahymena Cytoskeleton and Oral Apparatus
Release of the Cytosk