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Oxygen Radicals in Biological Systems, Part C

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ISBN-10: 012182134X

ISBN-13: 9780121821340

Edition: 1994

Authors: John N. Abelson, Melvin I. Simon, Helmut Sies, Helmut Sies

List price: $72.95
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Description:

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.
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Book details

List price: $72.95
Copyright year: 1994
Publisher: Elsevier Science & Technology
Publication date: 4/25/1994
Binding: Hardcover
Pages: 711
Size: 5.94" wide x 9.00" long x 1.50" tall
Weight: 2.464
Language: English

Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988;…    

Generation, Detection, and Characterization of Oxygen Radicals in Chemistry, Biochemistry, and Intermediate States of Reduction
Pulse Radiolysis
Pulse Radiolysis for Investigation of Nitric Oxide-Related Reactions
Sulfhydryl Free Radical Formation by Sonolysis, by Radiolysis, and Thermally: Vitamin A, Curcumin, Muconic Acid, and Related Conjugated Olefins and References
Suppression of Hydroxyl Radical Reactions in Biological Systems: Considerations Based on Competition Kinetics
Deoxyribose Assay for Detecting Hydroxyl Radicals
Detection of Hydroxyl Radicals by Aromatic Hydroxylation
Measurement of Iron and Copper in Biological Systems: Blemycin and Copper-Phenanthroline Assays
Oxygen Radicals Generation and DNA Scission by Anticancer and Synthetic Quinones
Spin Trapping of Hydroxyl Radicals in Biological Systems
In Vivo Detection of Radical Adducts by Electron Spin Resonance
Spin Trapping of Alcohol-Derived Radicals in Microsomes and Reconstituted Systemsby Electron Spin Resonance
In Vivo Visualization of Oxygen Radical-Dependent Photoemission
In Vivo Electron Paramagnetic Resonance Imaging of Skin
Electron Spin Resonance Assay of Ascorbate-Free Radicals in Vivo
Measurement of Superoxide Reaction by Chemiluminescence
Isolation, Characterization, and Assay of Enzymes or Substances Involved in Formation or Removal of Oxygen Radicals
Mass Spectrometry of Prostanoids: F2-Isoprostances Produced by NonCyclooxygenase Free Radical-Catalyzed Mechanism
Quantifying Aldehydes and Distinguishing Aldehydic Product Profiles from Autooxidation and Ozonation of Unsaturated Fatty Acids
Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hydroperoxides
Ferrylmyoglobin Formation: Chemical Reactivity Toward Electron-Donating Compounds
Enzymatic and Immunological Measurements of Soluable and Membrane-Bound Phospholipid-Hydroperoxide Glutathione Peroxidase
Targeting Superoxide Dismutase by Gene and Protein Engineering
Assays of Plasma and Membrane NADPH Oxidase
Oxidative Chemistry of Peroxynitrite
Nitric Oxide Assay Using Hemoglobin Method
Nitric-Oxide Synthase Assays
Isoforms of Nitric-Oxide Synthase: Purification and Regulation
Purification, Cloning, and Expression of Nitric-Oxide Synthase
Methods for Assessing Molecular, C