Forensic DNA Typing : Biology and Technology Behind STR Markers

ISBN-10: 012147951X
ISBN-13: 9780121479510
Edition: 2001
Authors: John M. Butler
List price: $79.95
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Description: Forensic DNA typing provides crime laboratories and legal professionals with the latest information on STR (short random repeat) markers that form the basis of the FBI and Europe-wide DNA database.

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Book details

List price: $79.95
Copyright year: 2001
Publisher: Elsevier Science & Technology Books
Publication date: 1/31/2001
Binding: Hardcover
Pages: 322
Size: 7.50" wide x 10.25" long x 0.75" tall
Weight: 2.134
Language: English

Forensic DNA typing provides crime laboratories and legal professionals with the latest information on STR (short random repeat) markers that form the basis of the FBI and Europe-wide DNA database.

John M. Butler is a NIST Fellow and Group Leader at the U.S. National Institute of Standards and Technology, Gaithersburg, Maryland. Dr. Butler earned his PhD from the University of Virginia while doing DNA research in the FBI Laboratory's Forensic Science Research Unit. He has over 100 publications in this field and is a frequent presenter on the topic of DNA typing.

Foreword
Introduction
Acknowledgements
Overview and History of DNA Typing
Biology
DNA Biology Review
Sample Collection and Preparation (DNA Extraction and Quantification)
The Polymerase Chain Reaction (DNA Amplification)
Commonly Used Short Tandem Repeat Markers
Biology of STRs: Stutter Products, Non-Template Addition, Microvariants, Null Alleles, and Mutation Rates
Forensic Issues: Degraded DNA, PCR Inhibition, Contamination, and Mixed Samples
Additional DNA Markers: Amelogenin, Y-Chromosome STRs, mtDNA, SNPs, Alu Repeats
Technology
DNA Separation Methods: Slab-Gel and Capillary Electrophoresis
DNA Detection Methods: Fluorescent Dyes and Silver Staining
The ABI Prism 310 Genetic Analyzer
The Hitachi Fmbio II Fluorescence Imaging System
STR Genotyping Issues
Laboratory Validation
New Technologies and Automation
Combined DNA Index System (Codis) and the Use of DNA Databases
Biology, Technology and Genetics
DNA Testing in High-Profile Cases
Reported STR Alleles: Sizes and Sequences
Ceph Family Study
DNA Advisory Board Quality Assurance Standards
Suppliers of DNA Analysis Equipment, Products, or Services
Index

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