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Biotechnology A Laboratory Course

ISBN-10: 0120845628
ISBN-13: 9780120845620
Edition: 2nd 1996 (Revised)
List price: $68.95 Buy it from $23.91
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Description: The objectives of this Second Editionof Biotechnology: A Laboratory Courseremain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a  More...

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Book details

List price: $68.95
Edition: 2nd
Copyright year: 1996
Publisher: Elsevier Science & Technology Books
Publication date: 3/1/1996
Binding: Paperback
Pages: 261
Size: 8.00" wide x 9.50" long x 0.50" tall
Weight: 1.100

The objectives of this Second Editionof Biotechnology: A Laboratory Courseremain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiaeand Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences.

Suggested Schedule for Laboratory Exercises
Introductory Notes
Record Keeping and Safety Rules
Format of Student Laboratory Records
The Ten Commandments of Record Keeping
Safety Rules in the Laboratory
Exercise 1: Aseptic Culture and Establishing Pure Cultures
Exercise 2: Preparation of Culture Media
Exercise 3: The Growth Curve
Exercise 4: Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep
Exercise 5: Purification, Concentration, and Quantitation of DNA. Exercise 6: Large-Scale Isolation of Plasmid DNA by Column Chromatography
Exercise 7: Amplification of a lacZ
Gene Fragment by the Polymerase Chain Reaction
Exercise 8: Restriction Digestion and Agarose Gel Electrophoresis
Exercise 9: Southern Transfer
Exercise 10: Preparation, Purification, and Hybridization of Probe
Exercise 11: Transformation of Saccharomyces cerevisiae
Exercise 12: Isolation of Plasmid from Yeast and Escherichia coli Transformation
Exercise 13: Protein Assays
Exercise 14: Qualitative Assay for Galactosidase in Yeast Colonies
Exercise 15: Determination of Galactosidase in Permeabilized Yeast Cells
Exercise 16: Assay of Galactosidase in Cell Extracts
Exercise 17: Galactosidase Purification
Exercise 18: Western Blot: Probe of Protein Blot with Antibody to Galactosidase
Appendix 1: Alternate Protocols and Experiments: Exercise 1A: Isolation and Characterization of Auxotrophic Yeast Mutants
Exercise 2A: Measurement of pH. Exercise 3A: Use of the Spectrophotometer
Exercise 6A: Isolation of Plasmid DNA: The Maxi-Prep
Exercise 10A: Colony Hybridization
: Buffer Solutions
Preparation of Buffers and Solutions
Properties of Some Common Concentrated Acids and Bases
Use of Micropipettors
Safe Handling of Microorganisms
List of Cultures
Storage of Cultures and DNA
Sterilization Methods
Preparation of Stock Solutions for Culture Media
Growth in Liquid Medium
Determination of Viable Cells
Determination of Cell Mass
Determination of Cell Number
Nomenclature of Strains
Glassware and Plasticware
Preparation of Tris and EDTA
Basic Rules for Handling Enzymes
Effects of Common Contaminants on Protein Assays
Manufacturers' and Distributors' Addresses
Surfing the Bionet: World Wide Web Addresses
Glossary
Subject Index

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