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    Biological Low-Voltage Scanning Electron Microscopy

    ISBN-10: 0387729704
    ISBN-13: 9780387729701
    Edition: 2008
    Author(s): Heide Schatten, James B. Pawley
    Description: Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing  More...
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    List Price: $229.00
    Copyright Year: 2008
    Publisher: Springer
    Publication Date: 12/18/2007
    Binding: Hardcover
    Pages: 317
    Size: 6.00" wide x 9.25" long x 0.75" tall
    Weight: 1.694
    Language: English

    Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

    Preface
    List of Contributors
    The Early Development of the Scanning Electron Microscope
    LVSEM for Biology
    The Aberration-Corrected SEM
    Noise and Its Effects on the Low-Voltage SEM
    High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols
    Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM
    Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM
    Cryo-SEM of Chemically Fixed Animal Cells
    High-Resolution and Low-Voltage SEM of Plant Cells
    High-Resolution Cryoscanning Electron Microscopy of Biological Samples
    Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy
    Index

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