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    Laser Manipulation of Cells and Tissues

    ISBN-10: 0123706483
    ISBN-13: 9780123706485
    Author(s): Michael W. Berns, Karl Otto Greulich
    Description: The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. This book ties all these systems and  More...
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    List Price: $180.00
    Publisher: Elsevier Science & Technology Books
    Binding: Hardcover
    Pages: 792
    Size: 7.50" wide x 9.25" long x 1.32" tall
    Weight: 3.432

    The use of laser-based manipulation tools has literally exploded on the cell biology and molecular pathology scene, resulting in as many different laser micromanipulation systems as there are people using them. This book ties all these systems and studies together, describing all of the different kinds of research and practical/analytical applications of laser manipulation. It also provides the reader with the basic information needed to actually build ones own laser micro-manipulation system. The combination of imaging and molecular probe technology with laser micromanipulation greatly extends the use of this technology in molecular, cellular, developmental and patho-biology/medicine. This book should be valuable to scientists, clinicians, and students in the fields of cell and developmental biology, cell physiology, cancer biology, pathology, and stem cell biology. * Devotes four chapters to laser catapulting and capture of DNA and other cellular material for biochemical analysis a major use of this technology that has been adapted for molecular pathology both in clinical medicine and research * Discusses the theory of laser tweezers (optical tweezers) and its application to novel problems in biology * Covers topics on optoporation (getting things into cells), uncaging of molecules, and the ability to collect and analyze nanomolar amounts of cell material by an array of biochemical/physical tools of particular interest to cell biologists and drug discovery researchers

    A History of Laser Scissors (Microbeams)
    Selected Applications of Laser Scissors and Tweezers and New Applications in Heart Research
    Mechanisms of Laser Cellular Microsurgery
    Cellular and Colloidal Separation using Optical Forces
    Laser Microsurgery in the GFP Era A Cell Biologists Perspective
    Investigating Relaxation Processes in Cells and Developing Organisms: From Cell Ablation to Cytoskeleton Nanosurgery
    Fs-Laser Scissors for Photobleaching, Ablation in Fixed Samples and Living Cells and Studies of Cell Mechanics
    Cellular Laserfection
    Chromophore-Assisted Laser Inactivation (CALI)
    Laser Surgery and Optical Trapping in a Laser Scanning Microscope
    Investigation of Laser Microdissected-Inclusion Bodies
    In situ Analysis of DNA Damage Response and Repair Using Laser Microirradiation
    Laser Effects in the Manipulation of Human Eggs and Embryos for In Vitro Fertilization
    Laser Microdissection-Based Analysis of Plant Sex Chromosomes
    Rapid Sampling for Single-Cell Analysis by Capillary Electrophoresis
    Physics of Optical Tweezers
    Laser Tweezers in the Study of Mechanobiology in Live Cells
    Optical Micromanipulation Methods for Controlled Rotation, Transportation and Microinjection of Biological Objects
    Optical Torque on Microscopic Objects
    Automated Motile Cell Capture and Analysis with Optical Traps
    Principles of Laser Microdissection and Catapulting of Histologic Specimens and Live Cells
    Non-contact Laser Microdissection and Catapulting for Pure Sample Capture
    Laser Capture Microdissection and Laser Pressure Catapulting as Tools to Study Gene Expression in Individual Cells of a Complex Tissue
    Laser Capture Microdissection in Comparative Proteomics Analysis of Hepatocellular Carcinoma
    Laser-based Measurements in Cell Biology
    Practical Aspects of Working with Laser Microbeams and Optical Tweezers

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