| |
| |
| Preface | |
| |
| |
| Contributors | |
| |
| |
| |
| Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils | |
| |
| |
| |
| |
| In Vitro Development of Human Killer-Immunoglobulin Receptor-Positive NK Cells | |
| |
| |
| |
| |
| Subset Analysis of Human and Mouse Mature NK Cells | |
| |
| |
| |
| |
| Assessing Licensing of NK Cells | |
| |
| |
| |
| |
| Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development In Vivo | |
| |
| |
| |
| |
| Use of Transfected Drosophila S2 Cells to Study NK Cell Activation | |
| |
| |
| |
| |
| Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry | |
| |
| |
| |
| |
| Studying NK Cell/Dendritic Cell Interactions | |
| |
| |
| |
| |
| Analysis of the NK Cell Immunological Synapse | |
| |
| |
| |
| |
| Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry | |
| |
| |
| |
| |
| Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells | |
| |
| |
| |
| |
| A Model System for Studying NK Cell Receptor Signaling | |
| |
| |
| |
| |
| Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction | |
| |
| |
| |
| |
| Lentiviral Gene Transduction in Human and Mouse NK Cell Lines | |
| |
| |
| |
| |
| Introduction of shRNAs into Human NK-Like Cell Lines with Retrovirus | |
| |
| |
| |
| |
| Introduction of shRNAs into Primary NK Cells with Lentivirus | |
| |
| |
| |
| |
| Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands | |
| |
| |
| |
| |
| Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation | |
| |
| |
| |
| |
| Identification of NK Cell Receptor Ligands Using a Signaling Reporter System | |
| |
| |
| |
| |
| Determining Ligand Specificity of Ly49 Receptors | |
| |
| |
| |
| |
| Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis | |
| |
| |
| |
| |
| A Simple Method to Measure NK Cell Cytotoxicity In Vivo | |
| |
| |
| |
| |
| Functional Analysis of Human NK Cells by Flow Cytometry | |
| |
| |
| |
| |
| Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry | |
| |
| |
| |
| |
| KIR Genotyping by Multiplex PCR-SSP | |
| |
| |
| |
| |
| Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes | |
| |
| |
| |
| |
| Use of Inbred Mouse Strains to Map Recognition Receptors of MCMV Infected Cells in the NK Cell Gene Locus | |
| |
| |
| |
| |
| Studying NK Cell Responses to Ectromelia Virus Infections in Mice | |
| |
| |
| |
| |
| Activation of Human NK Cells by Malaria-Infected Red Blood Cells | |
| |
| |
| |
| |
| Natural Killer Cells in Human Pregnancy | |
| |
| |
| |
| |
| Analysis of Uterine Natural Killer Cells in Mice | |
| |
| |
| |
| |
| Isolation of NK Cells and NK-Like Cells from the Intestinal Lamina Propria | |
| |
| |
| |
| Appendix | |
| |
| |
| Index | |