| |
| |
| Dedication | |
| |
| |
| Foreword | |
| |
| |
| Preface | |
| |
| |
| Color Plates | |
| |
| |
| Contributors | |
| |
| |
| Value Added eBook Instructions | |
| |
| |
| |
| Introduction | |
| |
| |
| |
| An Historical Perspective on the Clinical Diagnostic Laboratory | |
| |
| |
| |
| |
| Basic Molecular Biology | |
| |
| |
| |
| An Overview of Nucleic Acid Chemistry, Structure, and Function: The Foundations of Molecular Biology | |
| |
| |
| |
| |
| Extraction of Nucleic Acids | |
| |
| |
| |
| |
| Nucleic Acid Blotting Techniques: Theory and Practice | |
| |
| |
| |
| |
| The Polymerase Chain Reaction | |
| |
| |
| |
| |
| Bioinformatics: Computer-Based Approaches to Genetic Analysis | |
| |
| |
| |
| |
| Molecular Diagnostic Technologies | |
| |
| |
| |
| PCR-Based Methods for Mutation Detection | |
| |
| |
| |
| |
| Alternative Methods for Amplified Nucleic Acid Testing | |
| |
| |
| |
| |
| Electrophoretic Methods for Mutation Detection and DNA Sequencing | |
| |
| |
| |
| |
| Single-Nucleotide Polymorphisms: Testing DNA Variation for Disease Association | |
| |
| |
| |
| |
| Microarray Approaches to Gene Expression Analysis | |
| |
| |
| |
| |
| Methods for Analysis of DNA Methylation | |
| |
| |
| |
| |
| Other Clinical Diagnostic Technologies | |
| |
| |
| |
| Flow Cytometry | |
| |
| |
| |
| |
| Medical Cytogenetics | |
| |
| |
| |
| |
| Fluorescence In Situ Hybridization: A Major Milestone in Luminous Cytogenetics | |
| |
| |
| |
| |
| Immunohistochemistry | |
| |
| |
| |
| |
| Laser Capture Microdissection | |
| |
| |
| |
| |
| Quality Assurance in the Molecular Diagnostics Laboratory | |
| |
| |
| |
| Framework for Quality Assurance in Molecular Diagnostics | |
| |
| |
| |
| |
| Verification of Molecular Assays | |
| |
| |
| |
| |
| Standards and Standardization of Molecular Diagnostics | |
| |
| |
| |
| |
| Laboratory-Developed Tests in Molecular Diagnostics | |
| |
| |
| |
| |
| Applications of Molecular Diagnostics for Genetic Diseases | |
| |
| |
| |
| An Overview of Molecular Genetics | |
| |
| |
| |
| |
| Genetic Basis of Neurologic and Neuromuscular Diseases | |
| |
| |
| |
| |
| Molecular Mechanisms of Endocrine Disorders | |
| |
| |
| |
| |
| Molecular Pathogenesis of Cardiovascular Disease | |
| |
| |
| |
| |
| Molecular Diagnostics in Coagulation | |
| |
| |
| |
| |
| Cystic Fibrosis | |
| |
| |
| |
| |
| Prenatal Genotyping for Identification of Fetuses at Risk for Immune Cytopenic Disorders | |
| |
| |
| |
| |
| Personalized Medicine | |
| |
| |
| |
| |
| Applications of Molecular Diagnostics for Human Cancers | |
| |
| |
| |
| Molecular Pathogenesis of Human Cancer | |
| |
| |
| |
| |
| Application of Molecular Diagnostics to Hereditary Nonpolyposis Colorectal Cancer | |
| |
| |
| |
| |
| Molecular Genetic Applications to the Diagnosis of Lymphoma | |
| |
| |
| |
| |
| Molecular Genetic Abnormalities in Acute and Chronic Leukemias | |
| |
| |
| |
| |
| Applications of Molecular Diagnostics for Infectious Diseases | |
| |
| |
| |
| Molecular Testing for Chlamydia trachomatis and Neisseria gonorrhoeae | |
| |
| |
| |
| |
| Human Papillomavirus | |
| |
| |
| |
| |
| Molecular Diagnosis for HIV-1 | |
| |
| |
| |
| |
| Hepatitis C | |
| |
| |
| |
| |
| Cytomegalovirus | |
| |
| |
| |
| |
| Applications of Molecular Diagnostics for Identity-Based Testing | |
| |
| |
| |
| HLA Typing Using Molecular Methods | |
| |
| |
| |
| |
| Molecular Analysis for Forensic Casework and Parentage Testing | |
| |
| |
| |
| |
| Molecular Assessment of Bone Marrow Transplant Engraftment | |
| |
| |
| |
| |
| Use of DNA-Based Identity Testing for Specimen Identification | |
| |
| |
| |
| |
| Issues for the Clinical Molecular Pathology Laboratory | |
| |
| |
| |
| Genetic Counseling Considerations in Molecular Diagnosis | |
| |
| |
| |
| |
| Ethical, Social, and Legal Issues Related to Molecular Genetic Testing | |
| |
| |
| |
| Index | |