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Methods to Study Litter Decomposition A Practical Guide

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ISBN-10: 1402033486

ISBN-13: 9781402033483

Edition: 2005

Authors: Manuel A. S. Gra�a, Felix B�rlocher, Mark O. Gessner

List price: $279.99
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Description:

Decomposition of organic matter is a major ecosystem process involving an array of different organisms, including bacteria, fungi and invertebrates. The main objective of this book is to provide students and laboratory instructors at universities and professional ecologists with a broad range of established methods to study plant litter decomposition. Detailed protocols for direct use in the field or laboratory are presented in an easy to follow step-by-step format. A short introduction to each protocol reviews the ecological significance and principles of the technique and points to key references. Although most methods are described for freshwater ecosystems, many will work equally well…    
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Book details

List price: $279.99
Copyright year: 2005
Publisher: Springer
Publication date: 4/5/2005
Binding: Hardcover
Pages: 329
Size: 6.25" wide x 9.50" long x 0.75" tall
Weight: 1.606

Preface
Litter Dynamics
Litter Input
Leaf Retention
Manipulation of Stream Retentiveness
Coarse Benthic Organic Matter
Leaching
Leaf Mass Loss Estimated by Litter Bag Technique
Coarse Particulate Organic Matter Budgets
Leaf Chemical and Physical Properties
Determination of Total Nitrogen and Phosphorus in Leaf Litter
Total Protein
Free Amino Acids
Determination of Total Carbohydrates
Determination of Soluble Carbohydrates
Total Lipids
Total Phenolics
Radial Diffusion Assay for Tannins
Acid Butanol Assay for Proanthocyanidins (Condensed Tannins)
Proximate Lignin and Cellulose
Leaf Toughness
Microbial Decomposers
Techniques for Handling Ingoldian Fungi
Maintenance of Aquatic Hyphomycete Cultures
An Illustrated Key to the Common Temperate Species of Aquatic Hyphomycetes
Molecular Approaches to Estimate Fungal Diversity
Terminal Restriction Fragment Length Polymorphism (T-RFLP)
Molecular Approaches to Estimate Fungal Diversity
Denaturing Gradient Gel Electrophoresis (DGGE)
Sporulation of Aquatic Hyphomycetes
Ergosterol as a Measure of Fungal Biomass
Acetate Incorporation into Ergosterol to Determine Fungal Growth Rates and Production
Bacterial Counts and Biomass Determination by Epifluorescence Microscopy
Secondary Production and Growth of Litter-Associated Bacteria
Isolation of Cellulose-degrading Bacteria
Extraction and Quantification of ATP as a Measure of Microbial Biomass
Respirometry
Enzymatic Capabilities
Extractellular Fungal Hydrolytic Enzyme Activity
Cellulases
Viscosimetric Determination of Endocellulase Activity
Fluorometric Determination of the Activity of b-Glucosidase and other Extracellular Hydrolytic Enzymes
Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase
Lignin-Degrading Enzymes (Phenoloxidase and Peroxidase)
Phenol Oxidation
Proteinase Activity: Azocoll and Thin-Layer Enzyme Assay
Detritivorous Consumers
Maintenance of Shredders in the Laboratory
Feeding Preferences of Shredders
Data Analysis
A Primer for Statistical Analysis
Biodiversity