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Preface | |
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Litter Dynamics | |
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Litter Input | |
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Leaf Retention | |
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Manipulation of Stream Retentiveness | |
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Coarse Benthic Organic Matter | |
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Leaching | |
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Leaf Mass Loss Estimated by Litter Bag Technique | |
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Coarse Particulate Organic Matter Budgets | |
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Leaf Chemical and Physical Properties | |
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Determination of Total Nitrogen and Phosphorus in Leaf Litter | |
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Total Protein | |
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Free Amino Acids | |
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Determination of Total Carbohydrates | |
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Determination of Soluble Carbohydrates | |
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Total Lipids | |
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Total Phenolics | |
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Radial Diffusion Assay for Tannins | |
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Acid Butanol Assay for Proanthocyanidins (Condensed Tannins) | |
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Proximate Lignin and Cellulose | |
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Leaf Toughness | |
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Microbial Decomposers | |
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Techniques for Handling Ingoldian Fungi | |
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Maintenance of Aquatic Hyphomycete Cultures | |
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An Illustrated Key to the Common Temperate Species of Aquatic Hyphomycetes | |
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Molecular Approaches to Estimate Fungal Diversity | |
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Terminal Restriction Fragment Length Polymorphism (T-RFLP) | |
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Molecular Approaches to Estimate Fungal Diversity | |
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Denaturing Gradient Gel Electrophoresis (DGGE) | |
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Sporulation of Aquatic Hyphomycetes | |
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Ergosterol as a Measure of Fungal Biomass | |
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Acetate Incorporation into Ergosterol to Determine Fungal Growth Rates and Production | |
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Bacterial Counts and Biomass Determination by Epifluorescence Microscopy | |
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Secondary Production and Growth of Litter-Associated Bacteria | |
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Isolation of Cellulose-degrading Bacteria | |
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Extraction and Quantification of ATP as a Measure of Microbial Biomass | |
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Respirometry | |
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Enzymatic Capabilities | |
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Extractellular Fungal Hydrolytic Enzyme Activity | |
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Cellulases | |
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Viscosimetric Determination of Endocellulase Activity | |
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Fluorometric Determination of the Activity of b-Glucosidase and other Extracellular Hydrolytic Enzymes | |
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Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase | |
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Lignin-Degrading Enzymes (Phenoloxidase and Peroxidase) | |
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Phenol Oxidation | |
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Proteinase Activity: Azocoll and Thin-Layer Enzyme Assay | |
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Detritivorous Consumers | |
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Maintenance of Shredders in the Laboratory | |
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Feeding Preferences of Shredders | |
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Data Analysis | |
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A Primer for Statistical Analysis | |
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Biodiversity | |
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