Enzyme Kinetics and Mechanism

ISBN-10: 0815341407

ISBN-13: 9780815341406

Edition: 2007

Authors: Paul F. Cook, W. W. Cleland

List price: $93.00
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This textbook covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism - including acid-base chemistry and transition state structure. It features practical examples throughout, with numerous general experimental protocols and how-to-explanations.
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Book details

List price: $93.00
Copyright year: 2007
Publisher: Routledge
Publication date: 3/6/2007
Binding: Hardcover
Pages: 416
Size: 7.75" wide x 9.75" long x 1.00" tall
Weight: 1.892
Language: English

Paul F. Cook is the Grace B. Kerr Centennial Professor of Chemistry and Biochemistry at the University of Oklahoma. His awards and achievements twice included the Alexander von Humboldt Research Fellowship and he was the recipient of the National Institutes of Health Research Career Development Award from 1983 to 1988. Professor Cook is Associate Editor of Protein and Peptide Letters and Executive Editor of Proteins and Proteomics.W.W. Cleland is the M.J. Johnson Professor and Steenbock Professor of Chemical Science at the Enzyme Institute at University of Wisconsin-Madison. In the early 1960s, Cleland�s landmark papers created a theoretical framework for the study of steady-state kinetics of multisubstrate enzymes and laid the foundation for all subsequent steady-state studies. He serves on the editorial boards of Journal of Biological Chemistry and of Biochemistry. Recognized by many as the �father of modern enzyme kinetics,� Professor Cleland was elected to the National Academy of Sciences in 1985. 

Introduction to Kinetics
Enzyme Assays
Derivation of Initial Velocity Rate Equations and Data Processing
Initial Velocity Studies in the Absence of Added Inhibitors
Initial Velocity Studies: Presence of Added Inhibitors
Pre-Steady State and Relaxation Kinetics
Isotopic Probes of Kinetic Mechanism
Isotope Effects as a Probe of Mechanism
pH Dependence of Kinetic Parameters and Isotope Effects
King and Altman Patterns and Distribution Equations
Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations and Rate Constants for a Number of Multireactant Mechanisms
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