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Figures | |
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Color Plates | |
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Preface | |
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Abbreviations | |
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Introduction | |
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Background | |
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Advantages of Tissue Culture | |
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Limitations | |
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Major Differences In Vitro | |
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Types of Tissue Culture | |
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Biology of Cultured Cells | |
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The Culture Environment | |
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Cell Adhesion | |
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Cell Proliferation | |
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Differentiation | |
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Energy Metabolism | |
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Initiation of the Culture | |
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Evolution of Cell Lines | |
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The Development of Continuous Cell Lines | |
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Origin of Cultured Cells | |
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Design and Layout | |
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Planning | |
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Construction and Services | |
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Layout | |
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Equipment | |
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Requirements of a Tissue Culture Laboratory Essential Equipment | |
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Beneficial Equipment | |
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Useful Additional Equipment | |
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Consumable Items | |
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Aseptic Technique | |
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Objectives of Aseptic Technique | |
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Elements of Aseptic Environment | |
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Sterile Handling | |
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Laminar Flow | |
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Standard Procedure | |
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Apparatus and Equipment | |
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Safety | |
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Risk Assessment | |
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General Safety | |
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Fire | |
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Radiation | |
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Biohazards | |
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Culture Vessels | |
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The Substrate | |
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Choice of Culture Vessel | |
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Specialized Systems | |
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Treated Surfaces | |
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Media | |
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Development of Media | |
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Physicochemical Properties | |
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Balanced Salt Solutions | |
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Complete Media | |
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Serum | |
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Selection of Medium and Serum | |
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Other Supplements | |
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Serum-Free Media | |
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Disadvantages of Serum | |
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Advantages of Serum-Free Media | |
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Disadvantages of Serum-Free Media | |
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Replacement of Serum | |
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Selection of Serum-Free Medium | |
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Development of Serum-Free Medium | |
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Preparation of Serum-Free Medium | |
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Conclusions | |
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Preparation and Sterilization | |
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Apparatus | |
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Reagents and Media | |
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Control, Testing, and Storage of Media | |
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Primary Culture | |
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Types of Primary Cell Culture | |
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Isolation of the Tissue | |
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Primary Culture | |
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Cell Lines | |
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Nomenclature | |
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Subculture and Propagation | |
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Immortalization of Cell Lines | |
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Cell Line Designations | |
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Selection of Cell Line | |
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Routine Maintenance | |
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Cloning and Selection | |
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Cloning | |
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Stimulation of Plating Efficiency | |
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Suspension Cloning | |
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Isolation of Clones | |
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Replica Plating | |
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Selective Inhibitors | |
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Isolation of Genetic Variants | |
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Interaction with Susbstrate | |
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Cell Separation | |
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Cell Density and Isopyknic Sedimentation | |
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Antibody-Based Techniques | |
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Magnetic Sorting | |
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Cell Size and Sedimentation Velocity | |
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Centrifugal Elutriation | |
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Fluorescence-Activated Cell Sorting | |
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Other Techniques | |
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Beginner's Approach to Cell Separation | |
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Characterization | |
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The Need for Characterization | |
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Morphology | |
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Chromosome Analysis | |
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DNA Content | |
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RNA and Protein | |
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Enzyme Activity | |
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Antigenic Markers | |
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Differentiation | |
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Authentication | |
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Differentiation | |
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Expression of In Vivo Phenotype | |
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Stages of Commitment and Differentiation | |
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Proliferation and Differentiation | |
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Commitment and Lineage | |
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Markers of Differentiation | |
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Induction of Differentiation | |
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Differentiation and Malignancy | |
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Practical Aspects | |
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Transformation | |
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Role in Cell Line Characterization | |
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What is Transformation? | |
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Genetic Instability | |
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Immortalization | |
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Aberrant Growth Control | |
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Tumorigenicity | |
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Contamination | |
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Sources of Contamination | |
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Types of Microbial Contamination | |
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Monitoring for Contamination | |
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Eradication of Contamination | |
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Cross-Contamination | |
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Conclusions | |
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Cryopreservation | |
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Need for Cryopreservation | |
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Preservation | |
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Cell Banks | |
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Transporting Cells | |
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Quantitation | |
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Cell Counting | |
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Cell Weight | |
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DNA Content | |
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Protein | |
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Rates of Synthesis | |
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Preparation of Samples for Enzyme Assay and Immunoassay | |
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Cytometry | |
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Replicate Sampling | |
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Cell Proliferation | |
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Plating Efficiency | |
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Labeling Index | |
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Cell Cycle Time (Generation Time) | |
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Cell Migration | |
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Cytotoxicity | |
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Introduction | |
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In Vitro Limitations | |
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Nature of the Assay | |
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Anticancer Drug Screening | |
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Transformation | |
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Inflammation | |
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Specialized Cells | |
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Epithelial Cells | |
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Mensenchymal Cells | |
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Neuroectodermal Cells | |
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Hematopoietic Cells | |
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Gonads | |
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Tumor Cells | |
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Sampling | |
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Disaggregation | |
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Primary Culture | |
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Characterization | |
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Development of Cell Lines | |
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Selective Culture | |
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Specific Tumor Types | |
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Organotypic Culture | |
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Cell Interaction and Phenotypic Expression | |
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Organ Culture | |
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Histotypic Culture | |
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Filter-Well Inserts | |
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Cultures of Neuronal Aggregates | |
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Organotypic Culture | |
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Scale-Up | |
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Scale-up in Suspension | |
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Scale-up in Monolayer | |
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Specialized Techniques | |
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Lymphocyte Preparation | |
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Autoradiography | |
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Time-Lapse Recording | |
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Confocal Microscopy | |
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Cell Synchrony | |
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Culture of Amniocytes | |
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Culture of Cells from Poikilotherms | |
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Molecular Techniques | |
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Molecular Biology in Cell Culture | |
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In Situ Molecular Hybridization | |
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Somatic Cell Fusion | |
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Production of Monoclonal Antibodies | |
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DNA Transfer | |
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Problem Solving | |
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Slow Cell Growth | |
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Choice of Medium | |
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Unstable Reagents | |
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Purity of Constituents | |
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Plastics | |
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Glassware | |
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Microbial Contamination | |
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Chemical Contamination | |
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Primary Culture | |
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Cloning | |
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Differentiation | |
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Feeding | |
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Subculture | |
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Cross-Contamination | |
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Cryopreservation | |
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Granularity of Cells | |
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Cell Counting | |
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Viability | |
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In Conclusion | |
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Reagent Appendix | |
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Trade Index | |
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Glossary | |
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References | |
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Index | |