Flow Cytometry First Principles

Name: Flow Cytometry First Principles
Price: 50.59 USD
Availability: InStock
ISBN: 9780471382249

ISBN-10: 0471382248

ISBN-13: 9780471382249

Edition: 2nd 2001 (Revised)

Authors: Alice Longobardi Givan

List price: $100.95

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This second edition reflects the recent advances in flow cytometry and includes a new chapter on the staining of intracellular proteins, including cytokines. The new laser options and new fluorochromes are also discussed.

Book details

List price: $100.95
Edition: 2nd
Copyright year: 2001
Publisher: John Wiley & Sons, Incorporated
Publication date: 7/23/2001
Binding: Paperback
Pages: 304
Size: 6.30" wide x 9.27" long x 0.66" tall
Weight: 0.880
Language: English



Preface


Acknowledgments (First Edition)


Acknowledgments (Second Edition)



The Past as Prologue



Setting the Scene



Instrumentation: Into the Black Box


Illumination of the Stream


Centering Cells in the Illuminating Beam


Detection of Signals from Cells


Electronics



Information: Harnessing the Data


Data Storage


Data Analysis



Seeing the Light: Lasers, Fluorochromes, and Filters


General Theory


Lasers


Fluorochromes


Partitioning the Signal with Lenses, Filters, and Mirrors


Spectral Compensation



Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating


Cells from Blood


Staining for Surface Markers


Controls


Quantitation


Sensitivity


The Strategy of Gating


Gating on Fluorescence



Cells from Within: Intracellular Proteins


Methods for Permeabilizing Cells


Examples of Intracellular Staining



Cells from Within: DNA in Life and Death


Fluorochromes for DNA Analysis


Ploidy


Cell Cycle Analysis


Two-Color Analysis for DNA and Another Parameter


Chromosomes


Apoptosis


Necrosis



The Sorting of Cells


Sorting Theory


Characterization of Sorted Cells


Alternative Methods for Sorting


The Condition of Cells After Sorting



Disease and Diagnosis: The Clinical Laboratory


The Hematology Laboratory


The Pathology Laboratory


Solid Organ Transplantation


Comments



Out of the Mainstream: Research Frontiers


Functional Assays


The Aquatic Environment


Reporter Molecules


Microbiology


Molecular Biology


Multiplex Cytometry for Soluble Analytes


Reproductive Technology



Flowing On: The Future


General References


Glossary


Figure Credits


Index