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Practical HPLC Method Development

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ISBN-10: 047100703X

ISBN-13: 9780471007036

Edition: 2nd 1997 (Revised)

Authors: Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch

List price: $226.95
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.
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Book details

List price: $226.95
Edition: 2nd
Copyright year: 1997
Publisher: John Wiley & Sons, Incorporated
Publication date: 3/17/1997
Binding: Hardcover
Pages: 800
Size: 6.50" wide x 9.50" long x 1.80" tall
Weight: 3.080

Getting Started
Basics of Separation
Detection Sensitivity and Selectivity
Sample Preparation
The Column
Non-Ionic Samples: Reversed- and Normal-Phase HPLC
Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC
Gradient Elution
Systematic Approach to the Reversed-Phase Separation of Regular Samples
Computer-Assisted Method Development
Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds
Chiral Separations
Preparative HPLC Separation
Quantitation (Including Trace Analysis)
Completing the Method: Validation and Transfer