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Basic Bioscience Laboratory Techniques A Pocket Guide

ISBN-10: 0470743093

ISBN-13: 9780470743096

Edition: 2011

Authors: Philip L. R. Bonner, Alan J. Hargreaves

List price: $19.50
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Description:

This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further readingAll of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations.In short, this is a ls"must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.
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Book details

List price: $19.50
Copyright year: 2011
Publisher: John Wiley & Sons, Limited
Publication date: 3/4/2011
Binding: Paperback
Pages: 232
Size: 4.65" wide x 7.13" long x 0.54" tall
Weight: 0.308
Language: English

Preface
Glossary
Abbreviations
The preparation of solutions in bioscience research
Introduction
Concentration
Using balances to weigh out reagents
Practical considerations when making a 1.0 M solution
Dilutions and the use of pipettes
Water, acids and bases
Buffers
The equilibrium/dissociation constant (Ka) for an acid or base and the Henderson-Hasselbalch equation
Summary
Microscopy
Introduction
Microscopes - general principles
Principles of image formation
Light microscopy
Electron microscopy
Summary
Spectrophotometry
Introduction
The electromagnetic spectrum
The absorbance of light
Absorbtion spectrophotometry
The laws of the absorbance of light
The Beer Lambert law
Spectrophotometers
Applications of spectrophotometry in bioscience
Summary
Data analysis and presentation
Introduction
Statistical analysis of data: some key definitions
Distributions
Statistical comparison of data
Presentation, structure and organization of data in laboratory reports
Summary
The extraction and clarification of biological material
General introduction
Extraction
Extraction methods for animal and plant tissue
Extraction methods for bacteria
Clarification
Centrifugation techniques
Points of good practice in centrifugation
Summary
Electrophoresis of proteins and nucleic acids
General introduction
Separation of protein mixtures by gel electrophoresis
Other electrophoretic techniques applied to proteins
Separation of nucleic acids by gel electrophoresis
Applications of gel electrophoresis of nucleic acids
Summary
Chromatography
General introduction
The theory of chromatography
Factors to consider in chromatography
Methods used to elute samples in chromatography
Different types of chromatography and what properties can be used to separate molecules
Thin layer chromatography (TLC)
High pressure liquid chromatography (HPLC)
Gas liquid chromatography (GLC)
Ion exchange chromatography (IEX)
Size exclusion chromatography
Affinity chromatography
Summary
Cell culture techniques
Introduction
Growth and maintenance of cells in culture
Summary
Suggestions for further reading
Index