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| Preface | |
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| Glossary | |
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| Abbreviations | |
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| The preparation of solutions in bioscience research | |
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| Introduction | |
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| Concentration | |
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| Using balances to weigh out reagents | |
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| Practical considerations when making a 1.0 M solution | |
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| Dilutions and the use of pipettes | |
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| Water, acids and bases | |
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| Buffers | |
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| The equilibrium/dissociation constant (Ka) for an acid or base and the Henderson-Hasselbalch equation | |
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| Summary | |
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| Microscopy | |
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| Introduction | |
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| Microscopes - general principles | |
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| Principles of image formation | |
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| Light microscopy | |
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| Electron microscopy | |
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| Summary | |
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| Spectrophotometry | |
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| Introduction | |
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| The electromagnetic spectrum | |
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| The absorbance of light | |
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| Absorbtion spectrophotometry | |
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| The laws of the absorbance of light | |
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| The Beer Lambert law | |
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| Spectrophotometers | |
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| Applications of spectrophotometry in bioscience | |
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| Summary | |
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| Data analysis and presentation | |
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| Introduction | |
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| Statistical analysis of data: some key definitions | |
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| Distributions | |
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| Statistical comparison of data | |
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| Presentation, structure and organization of data in laboratory reports | |
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| Summary | |
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| The extraction and clarification of biological material | |
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| General introduction | |
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| Extraction | |
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| Extraction methods for animal and plant tissue | |
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| Extraction methods for bacteria | |
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| Clarification | |
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| Centrifugation techniques | |
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| Points of good practice in centrifugation | |
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| Summary | |
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| Electrophoresis of proteins and nucleic acids | |
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| General introduction | |
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| Separation of protein mixtures by gel electrophoresis | |
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| Other electrophoretic techniques applied to proteins | |
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| Separation of nucleic acids by gel electrophoresis | |
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| Applications of gel electrophoresis of nucleic acids | |
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| Summary | |
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| Chromatography | |
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| General introduction | |
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| The theory of chromatography | |
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| Factors to consider in chromatography | |
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| Methods used to elute samples in chromatography | |
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| Different types of chromatography and what properties can be used to separate molecules | |
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| Thin layer chromatography (TLC) | |
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| High pressure liquid chromatography (HPLC) | |
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| Gas liquid chromatography (GLC) | |
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| Ion exchange chromatography (IEX) | |
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| Size exclusion chromatography | |
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| Affinity chromatography | |
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| Summary | |
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| Cell culture techniques | |
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| Introduction | |
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| Growth and maintenance of cells in culture | |
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| Summary | |
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| Suggestions for further reading | |
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| Index | |