Protein NMR Spectroscopy Practical Techniques and Applications

Name: Protein NMR Spectroscopy Practical Techniques and Applications
Availability: OutOfStock
ISBN: 9780470721933

ISBN-10: 0470721936

ISBN-13: 9780470721933

Edition: 2011

Authors: Lu-Yun Lian, Gordon Roberts

List price: $89.95

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Book details

List price: $89.95
Copyright year: 2011
Publisher: John Wiley & Sons, Limited
Publication date: 7/22/2011
Binding: Hardcover
Pages: 368
Size: 6.93" wide x 9.72" long x 0.98" tall
Weight: 1.782
Language: English



List of Contributors


Introduction



References



Sample Preparation, Data Collection and Processing




Introduction



Sample Preparation



Data Collection



Data Processing



Isotope Labelling




Introduction



Production Methods for Isotopically Labelled Proteins



Uniform Isotope Labelling of Proteins



Selective Isotope Labelling of Proteins



Segmental Labelling



SAIL Methods



Concluding Remarks



Resonance Assignments




Introduction



Resonance Assignment of Unlabelled Proteins



15N-Edited Experiments



Triple Resonance



Side-Chain Assignments



Measurement of Structural Restraints




Introduction



NOE-Based Distance Restraints



Dihedral Restraints Derived from J-Couplings



Hydrogen Bond Restraints



Orientational Restraints



Chemical Shift Structural Restraints



Solution Scattering Restraints



Calculation of Structures from NMR Restraints




Introduction



Historical Development



Structure Calculation Algorithms



Automated NOE Assignment



Nonclassical Approaches



Fully Automated Structure Analysis



Paramagnetic Tools in Protein NMR




Introduction



Types of Restraints



What Metals to Use?



Paramagnetic Probes



Examples



Conclusions and Perspective



Structural and Dynamic Information on Ligand Binding




Introduction



Fundamentals of Exchange Effects on NMR Spectra



Measurement of Equilibrium and Rate Constants



Detecting Binding - NMR Screening



Mechanistic Information



Structural Information



Macromolecular Complexes




Introduction



Spectral Simplification through Differential Isotope Labelling



Basic NMR Characterisation of Complexes



3D Structure Determination of Macromolecular Protein-Ligand Complexes



Literature Examples



Studying Partially Folded and Intrinsically Disordered Proteins Using NMR Residual Dipolar Couplings




Introduction



Ensemble Descriptions of Unfolded Proteins



Experimental Techniques for the Characterisation of IDPs



NMR Spectroscopy of Intrinsically Disordered Proteins



Residual Dipolar Couplings



Conclusions


References


Index