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Handbook of Biological Confocal Microscopy

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ISBN-10: 0306448262

ISBN-13: 9780306448263

Edition: 2nd 1995

Authors: James B. Pawley

List price: $159.00
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This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.
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Book details

List price: $159.00
Edition: 2nd
Copyright year: 1995
Publisher: Springer
Publication date: 3/31/1995
Binding: Hardcover
Pages: 656
Size: 9.00" wide x 11.50" long x 1.25" tall
Weight: 4.444
Language: English

Foundations of Confocal Scanned Imaging in Light Microscopy
Fundamental Limits in Confocal Microscopy
Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM)
The Pixilated Image
Laser Sources for Confocal Microscopy
Non-Laser Light Sources
Objective Lenses for Confocal Microscopy
The Specimen Illumination Path and Its Effect on Image Quality
The Intermediate Optical System of Laser-Scanning Confocal Microscopes
Intermediate Optics in Nipkow Disk Microscopes
The Role of the Pinhole in Confocal Imaging System
Photon Detectors for Confocal Microscopy
The Collection, Processing, and Display of Digital Three-Dimensional Images of Biological Specimens
Visualization Systems for Multidimensional CLSM Images
Mapping and Measuring Surfaces Using Reflection Confocal Microscopy
Fluorophores for Confocal Microscopy
Image Contrast in Confocal Light Microscopy
Guiding Principles of Specimen Reservation for Confocal Fluorescence Microscopy
Confocal Microscopy of Living Cells
Lens Aberrations in Confocal Fluorescence Microscopy
Real-Time Stereo (3D) Confocal Microscopy
Signal-to-Noise in Confocal Microscopes
Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging
Light Microscopic Images Reconstructed by Maximum Likelihood Deconvolution
Direct View Confocal Imaging Systems Using a Slit Aperture
Two New High-Resolution Confocal Fluorescence Microscopies (4pi, Theta) with One- and Two-Photon Excitation
Optical Considerations at Ultraviolet Wavelengths in Confocal Microscopy
Two-Photon Molecular Excitation in Laser-Scanning Microscopy
Video-Rate Confocal Microscopy
Confocal Microscopy with Transmitted Light
Fluorescence Lifetime Imaging, A New Tool in Confocal Microscopy
Imaging Immunogold Labels with Confocal Microscopy
Fiberoptics in Confocal Microscopy
Comparison of Various Optical Sectioning Methods
Mass Storage and Hard Copy
Tutorial on Practical Confocal Microscopy and Use of the Confocal Test Specimen
Bibliography of Confocal Microscopes
Appendix I: Optical Units
Appendix 2: Light Paths of Current Commercial Confocal Light Microscopes for Biology