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Biological Electron Microscopy Theory, Techniques and Troubleshooting

ISBN-10: 0306442779

ISBN-13: 9780306442773

Edition: 1993

Authors: Michael J. Dykstra

List price: $97.00
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Description:

In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
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Book details

List price: $97.00
Copyright year: 1993
Publisher: Springer
Publication date: 11/30/1992
Binding: Hardcover
Pages: 380
Size: 7.00" wide x 10.25" long x 1.00" tall
Weight: 2.2
Language: English

Introduction
Specimen Preparation for Transmission Electron Microscopy
Fixatives
Buffers
Dehydration
Embedding Media
Examination of Four Tissues Prepared with a Variety of Fixatives and Buffers
A Quasi-Universal Fixation, Dehydration, and Embedment Schedule Successfully Used on Organisms from All Five Kingdoms of Life
Ultramicrotomy
Ultramicrotomes
Knives
Block Trimming
Sectioning Procedures
Eight Commonly Encountered Sectioning Problems
Support Films
Transmission Electron Microscopy
Historical Review of Microscopy (1590-1990s)
Theory of Electron Optics
Four Aspects of Image Formation
General TEM Features
Parts of the Electron Microscope: Functional Aspects
Operation of the TEM: Decision Making
Vacuum Systems
Types of Gauges
Vacuum Pumps
Sequential Operation of a Complete Vacuum System to Achieve High Vacuum
Lubrication of Vacuum Seals and Leak Detection
Staining Methods for Semithins and Ultrathins
Semithin Section Staining
Ultrathin Section Staining
Photography
Emulsion Composition
Film Types
Producing a Latent Image
Film Processing
Development Controls
Paper Types
Keeping Properties of Chemicals and Precautions
Sharpness
Films Used in the Electron Microscopy Laboratory
Copy Work
Types of Enlargers
Viewing a Print in Perspective
Replicas, Shadowing, and Negative Staining
Shadowing Casting
Negative Staining
Scanning Electron Microscopy
The Use of SEM in Biological Research and Medicine
Principles of the SEM
Operation of the SEM
Interaction of the Electron Beam and Specimen
Specimen Preparation
Artifacts and Their Correction
Specialty SEMS: FEG, LV, and ESEM Devices
Cryotechniques
Cryogens
Safety Precautions
Freezing Methods
Uses of Frozen Specimens
Artifacts and Their Correction
High-Voltage Electron Microscopy
Functional Aspects of HVEMS
Microscope Construction
Sample Preparation
Applications
Intermediate-Voltage Electron Microscopy (IVEM)
Microanalysis
Microanalysis Techniques
Cytochemistry
Problems
Specific Reaction Products
Examples of Enzyme Cytochemistry
Examples of Nonenzymatic Cytochemistry
Immunocytochemistry
Preparative Techniques
Immunoglobulins
Common Immunolabeling Techniques for Electron Microscopy
Autoradiography
Theoretical Aspects
Computer-Assisted Imaging
Resolution and Discrimination
Image Processing
Morphometric Analysis
Stereology
Computer-Assisted Analysis of Movement
Scanning Tunneling Microscopy and Its Derivatives
Appendix A: Laboratory Safety
Appendix B: Literature Sources for Electron Microscopy
Atlases
Journals
Society Publications
Book Series
NIH Resources for Intermediate Voltage Electron Microscopy (IVEM)
Appendix C: Electron Microscopy Equipment and Supplies
Expendable Supplies and Small Equipment
Electron Microscopes
Diamond Knives
High-Vacuum Pumps
Ultramicrotomes
Equipment for Cryotechniques
Sputter Coaters and Vacuum Evaporators
Index